Simultaneous gene editing and haploid induction
AssigneeSyngenta Participations AG
InventorsQiudeng Que, Timothy Kelliher
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Abstract
The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.
Claims15
A method of editing dicot genomic DNA, comprising:
- a) obtaining a first dicot plant comprising a mutation in a centromeric histone 3 (CENH3) gene and optionally a maize CENH3 tailswap transgene, wherein said first dicot plant expresses a DNA modification enzyme and optionally at least one guide nucleic acid;
- b) obtaining a second dicot plant, wherein the second dicot plant comprises the dicot plant genomic DNA which is to be edited;
- c) pollinating the first dicot plant with pollen from the second dicot plant;
- and
- d) selecting at least one haploid progeny produced by the pollination of step (c) wherein the haploid progeny comprises the genome of the second dicot plant but not the first dicot plant, and the genome of the haploid progeny has been modified by the DNA modification enzyme and optional at least one guide nucleic acid delivered by the first dicot plant;
- wherein the mutation in a CENH3 gene is selected from the group consisting of a loss-of-function mutation, a partial loss-of-function mutation, a restored frameshift mutation, and an in-frame deletion mutation.
Dependencies are inferred from the claim text.