Genome editing using Campylobacter jejuni CRISPR/CAS system-derived RGEN

A composition for genome editing of a target DNA sequence comprising:

• an effective amount of an isolated guide RNA comprising a first sequence forming a duplex with a complementary strand of the target DNA sequence and a second sequence interacting with a Cas protein that recognizes a proto-spacer-adjacent motif (PAM) sequence of NNNNRYAC (SEQ ID NO:
• 1) or a DNA encoding the isolated guide RNA, wherein the isolated guide RNA comprises a first region comprising the first sequence, and
• a second region comprising a stem-loop structure, wherein the stem-loop structure comprises a stem of 13-18 bp in length comprising a sequence of SEQ ID NO:
• 2 (5′-GUUUUAGUCCCUUGUG-3′) and a complementary sequence thereof, and
• a recombinant Cas protein that recognizes the PAM sequence of NNNNRYAC (SEQ ID NO:
• 1) or a nucleic acid encoding the recombinant Cas protein for performing genome editing of the target DNA sequence in a eukaryotic cell,
• wherein the target DNA sequence is derived from a genome of the eukaryotic cell, and
• wherein the target DNA sequence is adjacent to the PAM sequence of NNNNRYAC (SEQ ID NO:
• 1).