Back to results
US 10,519,439 B2Granted
High efficiency, small volume nucleic acid synthesis
AssigneesLife Technologies Corporation, Thermo Fisher Scientific Geneart GmbH
InventorsTodd Peterson, Axel Trefzer, Thomas Poehmerer
Life of this patent
Estimating the term…
Abstract
The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
Claims15
A method for producing a product nucleic acid molecule, the method comprising:
- (a) designing the product nucleic acid molecule;
- (b) synthesizing a plurality of nucleic acid molecules on separate beads in wells of a plate, wherein each well is configured to accommodate a single bead, wherein a subset of the nucleic acid molecules synthesized on the separate beads are components of the product nucleic acid molecule, wherein the volume of each well is between 0.1 picoliters and 10 nanoliters, wherein the wells comprise an electrode for the production of electrochemically generated acid, and wherein the individual nucleic acid molecules that are the components of the product nucleic acid molecule are capable of hybridizing with one or more of the other individual nucleic acid molecules that are the components of the product nucleic acid molecule;
- (c) selectively removing beads from the wells that comprise individual nucleic acid molecules that are the components of the product nucleic acid molecule, wherein beads comprising nucleic acid molecules that are not components of the product nucleic acid molecule are not removed from their respective wells;
- (d) collecting the beads selectively removed from wells in step (c) in a vessel;
- (e) releasing the nucleic acid molecules from the beads collected in the vessel in step (d) to form a pool of nucleic acid molecules in the vessel;
- and
- (f) assembling the product nucleic acid molecule from the pool of nucleic acid molecules formed in step (e).
4 more elements
Dependencies are inferred from the claim text.