Methods and compositions for generation of binding agents against cell surface antigens
AssigneeX-Body, Inc.
InventorsYan Chen, Steven M. Shamah
Life of this patent
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Abstract
Provided are methods and compositions for identifying binding polypeptides (e.g., antibodies or antigen binding fragments thereof) that specifically binds to a cell-surface antigen. The methods of the invention generally comprise contacting a variegated nucleic acid-display library of binding polypeptides with a cell-surface antigen displayed on the exterior surface of a cell; and isolating from the library at least one library member that specifically binds to the cell-surface antigen on the exterior surface of the cell. Also provided are novel nucleic acid display libraries (e.g., DNA display libraries) useful in the methods of the invention.
Claims17
A method of identifying a binding polypeptide that specifically binds to a cell-surface antigen, the method comprising:
- (a) contacting a cell, that naturally expresses a cell-surface antigen on its exterior surface, with a variegated DNA-display library of binding polypeptides, thereby forming a binding polypeptide/antigen complex, wherein the contacting of the cell with the DNA-display library is performed in suspension under conditions whereby the complex remains on the cell surface or is internalized;
- (b) washing unbound library members from the cell;
- (c) isolating at least one binding polypeptide/antigen complex;
- and
- (d) separating the at least one bound library member from the cell-surface antigen,
- thereby identifying a binding polypeptide that specifically binds to the cell-surface antigen.
Dependencies are inferred from the claim text.