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US 10,519,431 B2Granted
Thermostable variants of T7 RNA polymerase
AssigneeNew England Biolabs, Inc.
InventorsJennifer Ong, Vladimir Potapov, Kuo-Chan Hung, Haruichi Asahara, Shaorong Chong, George Tzertzinis
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Abstract
A bacteriophage RNA polymerase variant is provided. In some embodiments, the variant may have increased thermostability relative to the corresponding wild type bacteriophage RNA polymerase and/or wild type T7 RNA polymerase. Compositions, kits and methods that employ the variant are also provided.
Claims25
An RNA polymerase, wherein the RNA polymerase:
- (i) has at least 90% sequence identity to SEQ ID NO:
- 1;
- and
- (ii) comprises an amino acid substitution at the position corresponding to position 388 and at the position corresponding to position 567 of SEQ ID NO:
- 1.
2 more elements
Dependencies are inferred from the claim text.