Highly efficient method for establishing induced pluripotent stem cell
AssigneeKyoto University
InventorsShinya Yamanaka, Keisuke Okita
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Abstract
The present invention provides a production method of iPS cell, including a step of introducing the following (1) and (2): (1) an episomal vector containing a nuclear reprogramming factor; and (2) an episomal vector containing EBNA-1, which is different from (1), into a somatic cell, as well as a method for improving iPS cell establishment efficiency. The present invention also provides an agent for improving iPS cell establishment efficiency, which contains an episomal vector containing a nucleic acid encoding EBNA-1, and a kit for producing an iPS cell further containing an episomal vector containing a nucleic acid encoding a nuclear reprogramming factor.
Claims20
A method of producing a human iPS cell, comprising a step of introducing the following (1) and (2):
- (1) one or more episomal vectors containing a nucleic acid encoding a nuclear reprogramming factor, oriP, and a nucleic acid encoding EBNA-1;
- and
- (2) a plasmid vector containing a nucleic acid encoding EBNA-1 and not containing oriP
- into a human somatic cell, thereby producing a human iPS cell.
A method of producing a human iPS cell, comprising a step of introducing the following (1) and (2):
- (1) one or more episomal vectors containing a nucleic acid encoding a nuclear reprogramming factor, oriP, and a nucleic acid encoding EBNA-1;
- and
- (2) a plasmid vector containing a nucleic acid encoding EBNA-1 and not containing a replication origin functional in a mammalian cell
- into a human somatic cell, thereby producing a human iPS cell.
Dependencies are inferred from the claim text.