US 10,519,422 B2Granted

Method of producing human retinal pigment epithelial cells

AssigneeRiken

InventorsMasayo Takahashi, Satoshi Okamoto, Noriko Sakai

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Abstract

The invention relates to a method of producing a retinal pigment epithelial cell from a human pluripotent stem cell, and a method of treating or preventing a retinal disease by using the produced cell. The retinal pigment epithelial cell is prepared by (a) inducing differentiation of a human pluripotent stem cell into a pigment cell by adhesion cultivation of a human pluripotent stem cell in a medium containing a Nodal signal inhibitor and a Wnt signal inhibitor in the absence of a feeder cell to give a culture containing the pigment cell, (b) subjecting the obtained culture to further adhesion culture to give a culture containing a pigment cell colony, and (c) isolating the pigment cell from the obtained culture and culturing the cell to give a retinal pigment epithelial cell.

Claims7

Dependent claims

A method of producing a retinal pigment epithelial cell from a human pluripotent stem cell, which comprises

(a) a step of providing isolated human pluripotent stem cells, wherein the isolated human pluripotent stem cells are dispersed by a cell dissociation solution,
(b) a step of inducing differentiation of the isolated human pluripotent stem cells into pigment cells by adhesion cultivation of the isolated and dispersed human pluripotent stem cells of step (a) in a medium containing a Nodal signal inhibitor and a Wnt signal inhibitor at a concentration sufficient to induce differentiation of the human pluripotent stem cells into pigment cells in the absence of feeder cells to yield a culture containing the pigment cells,
(c) a step of subjecting the obtained culture of step (b) to further adhesion culture to yield a culture containing a pigment cell colony, and
(d) a step of isolating the pigment cell from the obtained culture of step (c) and culturing the cell to yield the retinal pigment epithelial cell.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 13/408,642Filed 29 Feb 2012
Published: US 2013/0224156 A129 Aug 2013
Granted: 31 Dec 2019
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12N 5/0621
Cells of the nervous system: eye cells, e.g. cornea, iris pigmented cells
A61K 35/30
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…
C12N 2501/115
Growth factors: basic fibroblast growth factor
C12N 2501/40
Active agents used in cell culture processes, e.g. differentation: regulators of development