Method for obtaining sterile human amniotic fluid and uses thereof
AssigneeMAM Holdings of West Florida, L.L.C.
InventorCarl Randall Harrell
Life of this patent
Estimating the term…
Abstract
Provided herein is a pre-Caesarean method for collecting amniotic fluid from a patient. A needle is inserted into the incision site for the future C-section, which may be under ultrasound guidance, through which the amniotic fluid is drawn under a low level suction and, optionally, gravity to a sterile collection container. The method encompasses filtering and/or irradiating the amniotic fluid to collect biomolecules of interest such as growth factors and/or stem cells. Also provided is the sterile amniotic fluid or filtrates thereof collected by the method described herein.
Claims14
A method of obtaining sterile filtered acellular non-irradiated human amniotic fluid comprising bioactive growth factors, the method comprising serially filtering the human amniotic fluid, the steps of the method comprising:
- collecting amniotic fluid under sterile conditions,
- removing cells and particulate matter from the human amniotic fluid by centrifuging or depth filtering the human amniotic fluid to obtain cell-free amniotic fluid, and
- serially filtering the cell-free amniotic fluid through multiple filters selected from the group consisting of filters with a pore size of 100 μm, 1.2 μm, 1.0 μm, 0.8 μm, 0.6, 0.45 μm, 0.22 μm and 0.2 μm to obtain a sterile filtered acellular human amniotic fluid without irradiation.
Dependencies are inferred from the claim text.