US 10,519,419 B2 · Method for producing culture medium composition

Abstract

The present invention provides a production method of a medium composition, including previously removing divalent metal cations contained in a polymer compound having an anionic functional group, preparing a dry powder of the polymer compound, and mixing the dry powder and the medium, and the like. The present invention also provides a purification method of a polymer compound having an anionic functional group, which includes mixing a suspension of the polymer compound having an anionic functional group in water and a cation exchanger that exchanges a divalent metal cation with a monovalent metal cation to give a polymer compound having an anionic functional group, which shows a reduced amount of contaminating divalent metal cations.

Claims13

Dependent claims

A method of producing a liquid medium composition capable of suspension culture of cells or tissues, comprising:

(a) subjecting deacylated gellan gum or a salt thereof to a divalent cation removal treatment;
(b) dissolving the deacylated gellan gum or a salt thereof subjected to the divalent cation removal treatment in water, without heating, to provide an aqueous solution at a concentration at which the deacylated gellan gum or a salt thereof before the divalent cation removal treatment is insoluble in water at room temperature;
(c) providing an aqueous solution of a culture medium;
and
(d) mixing the aqueous solution of deacylated gellan gum or a salt thereof and the aqueous solution of a culture medium,
thereby producing the liquid medium composition capable of suspension culture of cells or tissues.

3 more elements

Dependencies are inferred from the claim text.

Method for producing culture medium composition

Life of this patent

Method for producing culture medium composition

Life of this patent

Abstract

Claims13