Modified gram positive bacteria and uses thereof
AssigneeIntrexon Actobiotics NV
InventorsLothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
Life of this patent
Estimating the term…
Abstract
The present invention relates to gram positive bacteria with increased stress resistance and/or improved storage characteristics. In particular, the invention relates to gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack cellobiose-specific PTS system IIC component (PtcC)activity. The gram positive bacterium may further lack trehalose 6-phosphate phosphorylase (TrePP)activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.
Claims38
A method for improving a stress resistance or storage characteristic of a lactic acid bacterium (LAB) or Bifidobacterium, comprising a step of partially or completely deleting, disrupting, or inactivating an endogenous ptcC in the LAB or Bifidobacterium such that the LAB or Bifidobacterium is incapable of producing a functional PtcC;
- wherein the LAB or Bifidobacterium further comprises at least one feature selected from the group consisting of:
- (a) the LAB or Bifidobacterium constitutively overexpresses a gene encoding a trehalose transporter;
- (b) an endogenous trePP has been partially or completely deleted, disrupted, or inactivated such that the LAB or Bifidobacterium is incapable of producing a functional trehalose 6-phosphate phosphorylase (TrePP);
- and
- (c) the LAB or Bifidobacterium contains a functional heterologous trehalose 6-phosphatase gene.
Dependencies are inferred from the claim text.