Apparatus and method for continuous cell culture

A device for continuously culturing cells, comprising:

• a culture vessel having a compartment inside of which cells are capable of being cultured, the culture vessel comprising a top surface, a bottom surface and a side surface, the culture vessel being formed of a plastic material so as to be able to change a size of the compartment by an internal pressure or an external force;
• a sealing passageway formed in the culture vessel, the sealing passageway including a soft block through which the cells and a culture medium are capable of being injected into the compartment and cultured cells are capable of being removed from the compartment, using a syringe;
• a circulation filter formed in the culture vessel, the circulation filter including a conduit through which a gas required to culture the cells is able to be circulated into the compartment, a filter provided inside the conduit, and a clip provided around the conduit to selectively close or open the conduit;
• a scraper movably disposed on the bottom surface of the culture vessel, the scraper including a culture groove formed on a top surface of the scraper, a metal body, and a blade formed on a bottom surface of the scraper;
• and
• a moving member including a magnetic substance, the moving member being detachably attached to the scraper from outside of the culture vessel by the magnetic substance and the metal body, wherein, when the moving member moves, the scraper moves along the bottom surface of the culture vessel and scrapes the cultured cells by the blade of the scraper,
• wherein the culture vessel is sealed by the top surface, the bottom surface and the side surface when the conduit is closed by the clip, thereby being able to inject the cells and the culture medium into the compartment and remove the cultured cells from the compartment through the sealing passageway while continuously culturing the cells.