Stamp and method of transferring particles to cells of a cell culture
AssigneeWisconsin Alumni Research Foundation
InventorsDavid Beebe, Erwin Berthier, Mary Regier
Life of this patent
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Abstract
A stamp and a method for transferring particles to cells of a cell culture. The stamp includes a body having upper and lower surfaces. The lower surface includes a recessed portion. A gel extends along the recessed portion of the lower surface of the body and including the particles patterned therein. The body is configured to move between a first position wherein the gel is isolated from the cells of the cell culture and a second position wherein the particles patterned in the gel communicate with the cells of the cell culture through diffusion.
Claims15
A method of transmitting particles to cells of a cell culture, comprising the steps of:
- providing the cell culture on a surface;
- patterning the particles in a gel extending along a portion of a lower surface of a stamp;
- positioning the stamp with the particles patterned in the gel such that the particles in the gel extending along the lower surface of the stamp communicate with the cells of the cell culture;
- and
- allowing the particles in the gel to diffuse into the cells of the cell culture.
A method of transmitting particles to cells of a cell culture, comprising the steps of:
- forming a cell culture on a base;
- patterning the particles in a gel extending along a lower surface of a stamp;
- depositing cell culture media on the cell culture;
- positioning the stamp with the particles patterned in the gel in the cell culture media such that the particles in the gel extending along the lower surface of the stamp communicate with the cells of the cell culture;
- and
- allowing the particles in the gel to diffuse into the cells of the cell culture through the cell culture media.
Dependencies are inferred from the claim text.