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US 10,519,405 B2Granted
Stabilized alpha-amylase variants and use of the same
AssigneeNovozymes A/S
InventorsCarsten Andersen, Anna-Kathrine Fevre
Life of this patent
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Abstract
The present invention relates to alpha-amylase variants having an improved stability as compared to the parent alpha-amylase. The invention further relates to use of the variants, compositions comprising the variants, and methods of producing the variants.
Claims21
An alpha-amylase variant, wherein said variant has alpha-amylase activity and comprises an amino acid sequence that has at least 89% sequence identity to the amino acid sequence of SEQ ID NO:
- 1, wherein said variant comprises an amino acid motif selected from the group consisting of FRCK (SEQ ID NO:
- 11), FRYK (SEQ ID NO:
- 12), FSIK (SEQ ID NO:
- 13), FSLK (SEQ ID NO:
- 14), FRLK (SEQ ID NO:
- 15), FSQK (SEQ ID NO:
- 16) and FSSK (SEQ ID NO:
- 17) at the positions corresponding to amino acids 179 to 182 of SEQ ID NO:
- 2;
- and wherein said variant has improved stability in a detergent compared to an alpha-amylase comprising the amino acid sequence of SEQ ID NO:
- 2 with the amino acid motif FTGK (SEQ ID NO:
- 6) at the positions corresponding to amino acids 179 to 182 of SEQ ID NO:
- 2, wherein stability is determined by a PHADEBAS amylase assay.
10 more elements
Dependencies are inferred from the claim text.