Insecticidal polypeptides having broad spectrum activity and uses thereof
AssigneesPioneer Hi-Bred International, Inc., E. I. Du Pont de Nemours and Company
InventorsAndre Abad, Hua Dong, Janet Rice, Xiaomei Shi
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Abstract
The disclosure provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidopteran and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
Claims23
A variant Cry1J polypeptide comprising an amino acid sequence selected from SEQ ID NO:
- 4 and SEQ ID NO:
- 8 having a Valine substitution at position 578 compared to the native Cry1J polypeptide of SEQ ID NO:
- 2, wherein the variant Cry1J polypeptide has insecticidal activity against European corn borer, corn earworm, black cutworm, fall armyworm, Soybean looper and Velvet bean caterpillar, and increased stability against proteolytic degradation compared to the Cry1J polypeptide of SEQ ID NO:
- 2.
A variant Cry1J polypeptide comprising an amino acid sequence selected from SEQ ID NO:
- 4 and SEQ ID NO:
- 8 having a Valine substitution at position 578 compared to the native Cry1J polypeptide of SEQ ID NO:
- 2, wherein the variant Cry1J polypeptide has insecticidal activity against European corn borer, corn earworm, black cutworm, fall armyworm, Soybean looper and Velvet bean caterpillar, and increased stability against proteolytic degradation as measured in a Simulated Gastric Fluid (SGF) assay.
Dependencies are inferred from the claim text.