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US 10,519,196 B2Granted
System and method for producing interleukin receptor antagonist (IRA)
AssigneeEstar Technologies Ltd
InventorAaron Esteron
Life of this patent
Estimating the term…
Abstract
A system and method for producing high levels of autologous IL-1RA cytokine, includes a blood collection vessel, a cover, a portion of separation gel, an anticoagulant portion, a plasma collection syringe comprising a sharp needle, a buffy coat collection syringe and an incubation tube with cover. The blood collection vessel is adapted such that when containing whole blood and being centrifuged, after treatment yields separation fractions comprising, a first fraction of RBCs sediment, a second fraction of said gel, a third fraction comprising WBCs, platelets and a fourth fraction of plasma solution.
Claims15
A syringe system for producing autologous IL-1RA cytokine, comprising:
- a. a blood collection syringe;
- b. an anticoagulant portion;
- c. a syringe stopper;
- d. a sharp syringe needle;
- and
- e. an incubation vessel with cover and comprising washed and pretreated borosilicate glass beads in a size of about 0.5-5 mm;
- wherein said blood collection syringe includes the anticoagulant portion;
- further wherein said blood collection syringe is adapted such that, when containing whole blood, and centrifuged after treatment, yields separation fractions comprising:
- a first fraction comprising red blood cells (RBCs) sediment;
- a second fraction comprising white blood cells (WBCs) and platelets;
- and a third fraction comprising a plasma solution;
- and wherein said incubation vessel is adapted to incubate a portion of the second fraction comprising WBCs and a portion of the plasma solution for sufficient time and at a temperature to yield said autologous IL-1RA cytokine.
9 more elements
A vessel system for producing autologous IL-1RA cytokine, comprising:
- a. a blood collection syringe comprising a sharp needle and a filter unit;
- b. a filtered blood sample tube;
- c. a tube cover;
- d. a plasma portion collection syringe with a sharp needle;
- and
- e. an incubation vessel with a cover and comprising washed and pretreated borosilicate glass beads in a size of about 0.5-5 mm;
- wherein said blood collection syringe comprises a filter adapted to be placed in a downward position containing said whole blood, such that said whole blood is passed through said filter yielding plasma, platelets, and an RBC mixture, said filter adapted to retain a fraction of WBCs on or within said filter;
- wherein, when said filtered blood sample tube is centrifuged after treatment separation, fractions are yielded comprising:
- a first fraction comprising RBCs sediment;
- a second fraction comprising white blood cells (WBCs) and platelets;
- and a third fraction comprising a plasma solution;
- and
- wherein said filter comprising said second fraction of WBCs is adapted to be washed with said third fraction of plasma solution using said plasma portion collection syringe to yield a WBCs fraction.
10 more elements
A method for producing autologous IL-1RA cytokine in a closed system, the method comprising the steps of:
- a. obtaining a vacuum tube system for producing autologous IL-1RA cytokine, comprising:
- i. a blood collection vacuum tube;
- ii. a tube cover;
- iii. a portion of separation gel;
- iv. an anticoagulant portion;
- v. a plasma collection syringe comprising a sharp needle;
- and
- vi. an incubation tube with a cover and comprising washed and pretreated borosilicate glass beads in a size of about 0.5-5 mm;
- b. placing said anticoagulant portion and said portion of separation gel in said vacuum tube;
- said separation gel is adapted as a barrier and as a separating element between separation fractions;
- c. filling said blood collection vacuum tube with blood sample;
- d. separating by centrifugation there by obtaining separation fractions comprising:
- a first fraction comprising RBCs sediment;
- a second fraction comprising said separation gel;
- a third fraction comprising WBCs, platelets, and growth factors;
- and a fourth fraction comprising a plasma solution fraction;
- and
- e. discarding a portion of plasma with said plasma collection syringe comprising a sharp needle;
- f. extracting the remaining plasma and WBCs solution with said plasma collection syringe;
- g. transferring said solution into said incubation tube;
- and
- h. incubating said incubation tube;
- such that said autologous IL-1RA cytokine is obtained.
20 more elements
A method for producing autologous IL-1RA cytokine in a closed system, the method comprising the steps of:
- a. obtaining a syringe system for producing autologous IL-1RA cytokine, comprising:
- i. a blood collection syringe;
- ii. an anticoagulant portion;
- iii. a syringe stopper;
- iv. a sharp syringe needle;
- and
- v. an incubation vessel with a cover and comprising washed and pretreated borosilicate glass beads in a size of about 0.5-5 mm;
- b. placing the anticoagulant portion in the blood collection syringe;
- c. drawing whole blood with said blood collection syringe;
- d. positioning said syringe in a downward position comprising a stopper;
- e. separating said whole blood in said syringe by centrifugation yielding separation fractions comprising:
- a first fraction comprising RBCs sediment;
- a second fraction comprising WBCs and platelets;
- and a third fraction comprising a plasma solution;
- f. discarding RBCs fraction from said syringe;
- g. transferring the fraction of WBCs and platelets, growth factors and portion of plasma solution into incubation vessel;
- and
- h. incubating said incubation vessel;
- such that said autologous IL-1RA cytokine is obtained.
16 more elements
Dependencies are inferred from the claim text.