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US 10,519,194 B2Granted
Removal of fragments from a sample containing a target protein using activated carbon
AssigneeMerck Patent GmbH
InventorsMikhail Kozlov, Matthew T. Stone, Romas Skudas, Kevin Galipeau
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Abstract
The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal, of protein, fragments without adversely affecting the yield of the desired protein product.
Claims12
A method of reducing the amount of antibody fragments in a sample comprising an antibody to be purified, the method comprising the steps of:
- (a) providing a sample comprising an antibody and antibody fragments, wherein the fragments are in an amount equal to or greater than at least 0.2% of the amount of the antibody,
- (b) contacting the sample with activated carbon, wherein the activated carbon binds the antibody fragments,
- (c) removing the activated carbon from the sample,
- thereby resulting in reducing the amount of fragments in the sample.
1 more element
Dependencies are inferred from the claim text.