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US 10,519,189 B2Granted
Compositions and methods for synthesizing 5′-Capped RNAs
AssigneeTriLink BioTechnologies, LLC
InventorsRichard I. Hogrefe, Alexandre Lebedev, Anton P. McCaffrey, Dongwon Shin
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Abstract
Provided herein are methods and compositions for synthesizing 5′Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2′Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, “n” can be any integer from 0 to 4 and “m” can be an integer from 1 to 9.
Claims31
A complex comprising an initiating capped oligonucleotide primer and a DNA template,
- wherein the initiating capped oligonucleotide primer comprises a structure:
- wherein
- B1 and B2 are independently a natural, a modified, or an unnatural nucleoside base;
- and
- R1, R2, and R3 are independently OH or O-methyl;
- wherein the DNA template comprises a promoter region comprising a transcriptional start site having a first nucleotide at nucleotide position +1 and a second nucleotide at nucleotide position +2;
- and
- wherein the initiating capped oligonucleotide primer is hybridized to the DNA template at least at nucleotide positions +1 and +2.
5 more elements
Dependencies are inferred from the claim text.