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US 10,518,266 B2Granted
Microtiter plate and uses thereof
AssigneeOnexio Biosystems LLC
InventorsBrian Johnson, David Beebe, Jose Jimenez
Life of this patent
Estimating the term…
Abstract
Provided herein are microtiter plates, systems, and uses thereof. In particular, provided herein are microtiter plates with diffusion channels and their use in co-culture applications (e.g. in high throughput screening).
Claims13
A method of culturing cells, comprising:
- a) providing a device comprising:
- i) a plurality of first wells having a sidewall, an end wall, and a bottom wall, wherein a distance from said bottom wall of said first wells and an upper edge of said sidewall of said first wells defining a depth of said first wells;
- ii) a plurality of second wells, said plurality of second wells having a side wall, an end wall, and a bottom wall, wherein a distance from said bottom wall of said second wells and an upper edge of said sidewall of said second wells defining a depth of said second wells;
- wherein said second wells are adjacent with said first wells such that said end wall of said first wells is said end wall of said second wells;
- wherein said end wall connects with said bottom walls of said first and second wells to form a physical and fluidic barrier between said first and second wells;
- wherein at least a portion said end wall has a height less than said first and second depths, such that fluid in said first or second wells flows over said end wall when said fluid has a level exceeding said height and wherein fluid is prevented from moving between said first and second wells when said fluid has a level less than said height;
- b) placing a first cell into at least one of said first wells and a second cell into a second well adjacent therewith;
- c) adding culture media to said first and second wells at a depth lower than said height and culturing said cells for a first time period;
- and
- d) after said first time period, adding culture media to said first or second wells at a depth greater than said height and culturing said cells for a second time period under conditions such that small molecules are transferred between said first wells and said second wells.
7 more elements
A method of culturing cells, comprising:
- a) providing a device comprising:
- i) a plurality of first wells having a sidewall, an end wall, and a bottom wall, wherein a distance from said bottom wall of said first wells and an upper edge of said sidewall of said first wells defining a depth of said first wells;
- ii) a plurality of second wells, said plurality of second wells having a side wall, an end wall, and a bottom wall, wherein a distance from said bottom wall of said second wells and an upper edge of said sidewall of said second wells defining a depth of said second wells;
- wherein said second wells are adjacent with said first wells such that said end wall of said first wells is said end wall of said second wells;
- wherein said end wall connects with said bottom walls of said first and second wells to form a physical and fluidic barrier between said first and second wells;
- wherein at least a portion said end wall has a height less than said first and second depths, such that fluid in said first or second wells flows over said end wall when said fluid has a level exceeding said height and wherein fluid is prevented from moving between said first and second wells when said fluid has a level less than said height;
- wherein a capillary channel is positioned over said portion of said end wall that has a height less than said first and second depths;
- b) placing a first cell into at least one of said first wells and a second cell into a second well adjacent therewith;
- c) adding culture media to said first and second wells at a depth lower than said height and culturing said cells for a first time period;
- and
- d) after said first time period, adding culture media to said first or second wells at a depth that permits transfer of media between said first and second wells over said height and culturing said cells for a second time period under conditions such that small molecules are transferred between said first wells and said second wells.
8 more elements
Dependencies are inferred from the claim text.