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US 10,518,233 B2Granted
Phytase formulation
AssigneeBASF Enzymes LLC
InventorsYun Han, Michael Pratt, Yi Wu
Life of this patent
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Abstract
A liquid enzyme formulation, an enzyme granule formulation, methods for manufacturing enzyme granules using a fluid bed dryer, wherein the enzyme granules are thermostable without the need for a thermostable coating is provided. The enzyme granules are phytase granules used in the manufacturing of an animal feed, wherein the phytase granule is thermostable without the need for a thermostable coating and the phytase retains about 63% to about 134% of its activity after pelleting at 80° C.
Claims11
A liquid enzyme formulation comprising:
- (a) a phytase at a concentration of 5,000 to 50,000 unit/ml of the liquid enzyme formulation;
- (b) a buffer, at a concentration of 0.5%-2.5% w/v, wherein the buffer is selected from the group consisting of:
- a sodium citrate, potassium citrate, citric acid, sodium acetate, acetic acid, sodium phosphate, potassium phosphate and any combination thereof;
- (c) a stabilizer, at a concentration of 1%-40% w/v, wherein the stabilizer is selected from the group consisting of:
- a sucrose, a sorbitol, a mannitol, a glycerol, a trehalose, a sodium chloride, a sodium sulphate, or any combination thereof;
- and,
- (d) an anti-microbial, at a concentration of 0.05%-0.40% w/v, wherein the anti-microbial is selected from the group consisting of:
- a potassium sorbate, sodium sorbate, a sorbic acid, a sodium benzoate, a benzoic acid, a methyl paraben, a calcium propionate, a sodium propionate, an ammonium propionate, a propionic acid, or any combination thereof,
- wherein the phytase retains at least 63% of its activity at a temperature of 80° C.
6 more elements
Dependencies are inferred from the claim text.