Covalently linked antigen-antibody conjugates
AssigneeHoffman-La Roche Inc.
InventorsJoerg Benz, Ulrich Brinkmann, Stefan Dengl, Sebastian Dziadek, Guy Georges, Michael Grote, Alexander Haas, Eike Hoffmann
Life of this patent
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Abstract
Herein is reported a conjugate comprising an antigen and an antibody that specifically binds to the antigen wherein a covalent bond is formed between the antigen and an amino acid residue in the CDR2 of the antibody, an antibody comprising a cysteine residue at amino acid position 53 (according to Kabat) in the heavy chain CDR2 and uses thereof.
Claims16
An isolated antibody that binds to biotin, wherein the antibody comprises a heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO:
- 57;
- a heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO:
- 58;
- a heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO:
- 59;
- a light chain CDR1 comprising the amino acid sequence of SEQ ID NO:
- 61;
- a light chain CDR2 comprising the amino acid sequence of SEQ ID NO:62;
- and a light chain CDR3 comprising the amino acid sequence of SEQ ID NO:
- 63.
An isolated antibody that binds to theophylline, wherein the antibody comprises a heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO:
- 89;
- a heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO:
- 90;
- a heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO:
- 91;
- a light chain CDR1 comprising the amino acid sequence of SEQ ID NO:
- 93;
- a light chain CDR2 comprising the amino acid sequence of SEQ ID NO:94;
- and a light chain CDR3 comprising the amino acid sequence of SEQ ID NO:
- 95.
An isolated antibody that binds to fluorescein, wherein the antibody comprises a heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO:
- 105;
- a heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO:
- 106;
- a heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO:
- 107;
- a light chain CDR1 comprising the amino acid sequence of SEQ ID NO:
- 109;
- a light chain CDR2 comprising the amino acid sequence of SEQ ID NO:110;
- and a light chain CDR3 comprising the amino acid sequence of SEQ ID NO:
- 111.
An isolated antibody that binds to digoxigenin, wherein the antibody comprises a heavy chain CDR1 comprising the amino acid sequence of SEQ ID NO:
- 25;
- a heavy chain CDR2 comprising the amino acid sequence of SEQ ID NO:
- 26;
- a heavy chain CDR3 comprising the amino acid sequence of SEQ ID NO:
- 27;
- a light chain CDR1 comprising the amino acid sequence of SEQ ID NO:
- 29;
- a light chain CDR2 comprising the amino acid sequence of SEQ ID NO:30;
- and a light chain CDR3 comprising the amino acid sequence of SEQ ID NO:
- 31.
Dependencies are inferred from the claim text.