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AssigneesServicio Galego de Saude (Sergas), FundacióN Profesor Novoa Santos
InventorsGermán Bou Arévalo, María Clara Póvoa Cabral, Astrid Pérez Gómez, María Merino Carballeira, Alejandro Beceiro Casas, Patricia García Fernández
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The present invention refers to a method for the production of live attenuated bacterial strains, suitable as vaccine candidates, comprising the steps of: A. providing a bacterial strain capable of expressing glutamate racemase and possibly D-amino acid transaminase and comprising a peptidoglycan cell wail, and B. inactivating the gene or genes encoding for the glutamate racemase enzyme and, if needed, the gene or genes encoding for the enzyme D-amino acid transaminase in such way that the bacterial strain is no longer capable of expressing a functional glutamate racemase and/or a functional D-amino acid transaminase; wherein the inactivation of said genes causes said bacterial strain to be auxotrophic for D-glutamate.
A method of vaccinating a warm blooded animal or a human in need thereof against an infection caused by Pseudomonas aeruginosa, said method comprising administering to said warm blooded animal or said human a pharmaceutical composition comprising a pharmaceutically acceptable carrier or a diluent and an immunogenically effective amount of a live attenuated mutant of Pseudomonas aeruginosa, wherein said mutant is glutamate racemase deficient and is auxotrophic for D-glutamate, wherein said administering protects said warm blooded animal or said human against said infection.
A method of protecting a warm blooded animal or a human in need thereof against an infection caused by Pseudomonas aeruginosa, said method comprising administering to said warm blooded animal or said human a pharmaceutical composition comprising a pharmaceutically acceptable carrier or a diluent and an immunogenically effective amount of a live attenuated mutant of Pseudomonas aeruginosa, wherein said mutant is glutamate racemase deficient and is auxotrophic for D-glutamate, wherein said administering protects said warm blooded animal or said human against said infection.
Dependencies are inferred from the claim text.