Vaccines with higher carbohydrate antigen density and novel saponin adjuvant
AssigneeObi Pharma, Inc.
InventorsWei Han Lee, Nan-Hsuan Wang, Chung Hao Chang, Yih-Huang Hsieh, Cheng Der Tony Yu, Cheng-Chi Wang, Yu-Hsin Lin, Yu-Chen Lin, I-Ju Chen
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Abstract
The present invention provides vaccines comprising carbohydrate antigen conjugated to a diphtheria toxin (DT) as a carrier protein, wherein the ratio of the number of carbohydrate antigen molecule to the carrier protein molecule is higher than 5:1. Also disclosed herein is a novel saponin adjuvant and methods to inhibit cancer cells, by administering an effective amount of the vaccine disclose herein.
Claims16
A vaccine, comprising a glycoconjugate that comprises:
- (a) a carbohydrate antigen;
- and
- (b) an inactivated diphtheria toxin (DT),
- wherein each carbohydrate antigen is directly attached to the inactivated DT,
- wherein the epitope ratio of the carbohydrate antigen to the DT ranges from 5:1 to 39:1, and the carbohydrate antigen is Globo H hexasaccharide (Fucα1→2Galβ1→3GalNAcβ1→3Galα1→4Galβ1→4Glcβ1),
- wherein the vaccine is produced by incubating the carbohydrate antigen with DT in a basic pH1 condition.
A pharmaceutical composition, comprising a glycoconjugate that comprises:
- (a) a carbohydrate antigen;
- (b) an inactivated diphtheria toxin (DT);
- and
- (c) a pharmaceutically acceptable carrier,
- wherein each carbohydrate antigen is directly attached to the inactivated DT,
- wherein the epitope ratio of the carbohydrate antigen to the DT ranges from 5:1 to 39:1, and wherein the carbohydrate antigen is Globo H hexasaccharide (Fucα1→2Galβ1→3GalNAcβ1→3Galα1→4Galβ1→4Glcβ1).
A vaccine, comprising
- (a) a Globo H hexasaccharide (Fucα1→2Galβ1→3GalNAcβ1→3Galα1→4Galβ1→4Glcβ1);
- and
- (b) an inactivated diphtheria toxin (DT),
- wherein each carbohydrate antigen is directly attached to the inactivated DT,
- wherein the epitope ratio of Globo H hexasaccharide to DT ranges from 8:1 to 24:1, and wherein the vaccine is produced by incubating Globo H with DT in a basic pH condition.
Dependencies are inferred from the claim text.