US 10,517,903 B2 · Amnion derived therapeutic composition and process of making same

Abstract

Acellular amnion derived therapeutic compositions are described having a number of various compositional embodiments. An acellular amnion derived therapeutic composition has essentially no live or active amniotic cells. The amniotic cells may be destroyed and the cells and cell debris may be removed from the acellular amnion derived therapeutic composition. An acellular amnion derived therapeutic composition may comprise micronized placental tissue particles, and/or amniotic fluid. An acellular amnion derived therapeutic composition may be a dispersion of micronized amniotic membrane combined with a fluid, such as plasma, saline, amniotic fluid, combinations thereof and the like. An acellular amnion derived therapeutic composition may be combined with a matrix component to form a composite. An acellular amnion derived therapeutic composition may be used in conjunction with a composition comprising viable cells, such as stem cells.

Claims21

Dependent claims

A process for the formation of a therapeutic composition comprising the steps of:

a) providing an amniotic fluid from a mammalian donor comprising amniotic fluid cells, growth factors and proteins;
b) acellularizing said amniotic fluid to produce an acellular amniotic fluid,
wherein the acellular amniotic fluid has no more than about 750,000 intact cells per ml of said acellular amniotic fluid and wherein no more than 1% of the total cells present are viable;
c) filtering said acellular amniotic fluid to remove debris, thereby producing a filtered therapeutic composition;
d) adding a plurality of non-amnion derived viable cells to the filtered therapeutic composition;
e) subsequently cryopreserving said filtered therapeutic composition to produce a cryopreserved therapeutic composition;
and
f) thawing out said cryopreserved therapeutic composition to produce a thawed therapeutic composition having a plurality of non-amnion derived viable cells.

5 more elements

Dependencies are inferred from the claim text.

Amnion derived therapeutic composition and process of making same

Life of this patent

Amnion derived therapeutic composition and process of making same

Life of this patent

Abstract

Claims21