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US 10,517,890 B2Granted
Triggering RNA interference with RNA-DNA and DNA-RNA nanoparticles
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsBruce A. Shapiro, Kirill A. Afonin, Mathias D. Viard
Life of this patent
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Abstract
The instant invention provides RNA nanocubes, DNA nanocubes and R/DNA chimeric nanocubes comprising one or more functionalities. The multifunctional RNA nanocubes are suitable for therapeutic or diagnostic use in a number of diseases or disorders.
Claims18
A DNA nanoparticle that is capable of becoming activated for RNAi activity, comprising:
- a six-stranded DNA oligonucleotide nanocube with three single-stranded thymines at each corner of the nanocube, the DNA oligonucleotides being capable of self-assembling to form the DNA oligonucleotide nanocube, wherein one to six of the DNA oligonucleotides of the DNA oligonucleotide nanocube comprises only one single strand R/DNA arm covalently attached thereto;
- one to six cognate single strand R/DNA molecule(s), each of the cognate single strand R/DNA molecule(s) being capable of annealing at least one of the one to six R/DNA single strand arm(s) to form R/DNA hybrid arm(s);
- wherein the DNA oligonucleotide nanocube becomes activated for RNAi activity upon association with free R/DNA hybrid molecule(s) that present strand(s) capable of annealing to corresponding DNA and RNA strand(s) of the R/DNA hybrid arm(s) to form RNA/RNA or DNA/DNA hybrid arm(s) and free RNA/RNA or DNA/DNA hybrid molecule(s).
A RNA nanoparticle that is capable of becoming activated for RNAi activity, comprising:
- a six-stranded RNA oligonucleotide nanocube with three single-stranded uracils at each corner of the nanocube, the RNA oligonucleotides being capable of self-assembling to form the RNA oligonucleotide nanocube, wherein one to six of the RNA oligonucleotides of the RNA oligonucleotide nanocube each comprises only one single strand R/DNA arm covalently attached thereto;
- one to six cognate single strand R/DNA molecule(s), each of the cognate single strand R/DNA molecule(s) being capable of annealing to at least one of the one to six R/DNA single strand arm(s) to form R/DNA hybrid arm(s);
- wherein the RNA oligonucleotide nanocube becomes activated for RNAi activity upon association with free R/DNA hybrid molecule(s) that present strand(s) capable of annealing to corresponding DNA and RNA strand(s) of the R/DNA hybrid arm(s) to form RNA/RNA or DNA/DNA hybrid arm(s) and free RNA/RNA or DNA/DNA hybrid molecule(s).
Dependencies are inferred from the claim text.