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US 10,517,244 B2Granted
Method to develop herbicide-resistant sugar beet plants
AssigneeSESVanderHave NV
InventorsGuy Weyens, Marc Lefebvre, Rudiger Hain, Gerhard Johann
Life of this patent
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Abstract
A method for producing herbicide-resistant sugar beet plant includes obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant. A composition having an ALS herbicide is applied to the cells at a concentration which is lethal to the cells. Sugar beet plants are regenerated from the surviving cells.
Claims21
A method for producing a mutant sugar beet plant resistant to one or more inhibitor(s) of the acetohydroxyacid synthase enzyme (ALS) comprising the steps of:
- comparing a plurality of different sugar beet plant genotypes for the capacity of their stomatal guard cells protoplasts to regenerate into a sugar beet plant;
- selecting one of the plurality of different sugar beet plant genotypes having stomatal guard cells protoplasts with a probability of more than 10% to regenerate into viable sugar beet callus and more than 10% of calli obtained by said protoplasts having a capacity to develop shoots, wherein the one of the plurality of different sugar beet plant genotypes comprises the 4D genotype sugar beet plant, wherein a representative sample of the 4D genotype sugar beet seed has been deposited under NCIMB 43423;
- obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant of the selected genotype;
- applying to an in vitro culture of more than 20,000,000 of said protoplasts a composition comprising one or more ALS inhibitor(s) at a concentration that is lethal to more than 99% of the in vitro cultured cells;
- and
- regenerating a sugar beet plant from the surviving cells of said in vitro cultured cells, wherein the regenerated sugar beet plant is a mutant sugar beet plant resistant to one or more ALS inhibitor(s).
3 more elements
A method for producing a mutant sugar beet plant being resistant to an herbicide comprising the steps of:
- comparing a plurality of different sugar beet plant genotypes for the capacity of their stomatal guard cells protoplasts to regenerate into a sugar beet plant;
- selecting one of the plurality of different sugar beet plant genotypes having stomatal guard cells protoplasts with a probability of more than 10% to regenerate into viable sugar beet callus and more than 10% of calli obtained by said protoplasts having a capacity to develop shoots, the one of the plurality of different sugar beet plant genotypes is the 4D genotype sugar beet plant or a sugar beet plant having the same regenerating properties as the 4D genotype, wherein a representative sample of the 4D genotype sugar beet seed has been deposited under NCIMB 43423;
- obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant of the selected genotype;
- applying to an in vitro culture of more than 20,000,000 of said protoplasts a composition comprising said herbicide at a concentration that is lethal to more than 99% of the in vitro cultured cells;
- regenerating sugar beet plants from the surviving cells of said in vitro cultured cells;
- and
- selecting regenerated sugar beet plants having a mutation in the gene encoding the peptide targeted by said herbicide.
4 more elements
Dependencies are inferred from the claim text.