Method and device for two-dimensional separation of ionic species
AssigneeUniversitäT Regensburg
InventorsFrank-Michael Matysik, Andrea Beutner, Jonas Mark, Sven Kochmann
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Abstract
The invention relates to a method which realizes a two-dimensional separation of ionic species on the basis of the online coupling of ion chromatography (IC) and capillary electrophoresis (CE). A device for IC×CE coupling, its implementation in terms of two alternatives, the connection to a mass spectrometric detector, and corresponding application are described.
Claims12
Device for continuous two-dimensional separation of ionic species comprising
- a) an ion chromatography (IC) system, comprising a suppressor;
- b) a capillary electrophoresis (CE) system comprising an electrolyte vessel, a high voltage electrode, and a CE separation capillary;
- and
- c) a modulator, for transferring effluent of the IC system to the CE system, comprising a transfer capillary and injector means, wherein the injector means are adapted to provide discrete volume segments of effluent, wherein the injector means comprises a positioning and guidance system for modifying the distance between the outlet of the transfer capillary and the inlet of the CE separation capillary in a controlled manner, wherein the CE separation capillary or the transfer capillary or both are configured for movement in an axial direction.
Method for two-dimensional separation of ionic species by online coupling of ion chromatography (IC) and capillary electrophoresis (CE), comprising the following steps:
- a) injecting a sample into an IC system comprising a suppressor;
- b) transferring IC effluent through a transfer capillary to a CE system comprising an electrolyte vessel with electrophoresis buffer, a separation capillary and a high voltage electrode;
- and
- c) after step b) injecting volume segments of effluent to a separation capillary of the CE system via injector means,
- wherein the distance between the outlet of the transfer capillary and the inlet of the separation capillary of the CE system is periodically modified between a first position and a second position by movement of one or both capillaries, wherein the first position provides for a distance of more than 150 μm and the second position provides for a distance of less than 100 μm, wherein the movement is controlled by a modulator comprising a positioning and guidance system, wherein the movement of the CE separation capillary or the transfer capillary or both is in an axial direction.
Dependencies are inferred from the claim text.