Cell measurement method
AssigneeKurashiki Boseki Kabushiki Kaisha
InventorsYuko Nanjo, Hiroyuki Asano, Isao Miyagawa, Yoshio Takada
Life of this patent
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Abstract
To show a highly accurate cell measurement method. A cell measurement method comprises: a step of staining a cultured target cell with a dye; a step of obtaining a first image and a second image which are transmission images for a first light and a second light to which the dye has different absorbance; a step of dividing each of the first image and the second image into a plurality of divided regions and comparing the first image and the second image for each of the divided regions so as to eliminate noises; and a step of integrating an indicator of a cell amount in each of the divided regions in the images from which the noises were eliminated so as to evaluate a target cell amount.
Claims13
A cell measurement method, comprising:
- a step of staining a cultured target cell with a dye, wherein the target cell is a cell cultured by embedding the cell in a collagen gel;
- a step of obtaining a first image and a second image which are transmission images for a first light and a second light to which the dye has different absorbance;
- a step of dividing each of the first image and the second image into a plurality of divided regions and comparing the first image and the second image for each of the divided regions so as to eliminate noises;
- and
- a step of integrating an indicator of a cell amount in each of the divided regions in the images from which the noises were eliminated so as to evaluate a target cell amount.
A cell measurement method, comprising:
- a step of staining a cultured target cell with a dye;
- a step of obtaining a first image and a second image which are transmission images for a first light and a second light to which the dye has different absorbance;
- a step of dividing each of the first image and the second image into a plurality of divided regions and comparing the first image and the second image for each of the divided regions so as to eliminate noises;
- and
- a step of integrating an indicator of a cell amount in each of the divided regions in the images from which the noises were eliminated so as to evaluate a target cell amount,
- wherein the target cell amount is evaluated by calculating an absorbance from an image luminosity for each of the divided regions, and integrating the obtained absorbance over the plurality of divided regions to calculate an estimated volume value of the target cell.
Dependencies are inferred from the claim text.