Back to results
US 10,513,719 B2Granted
Engineered beta-subunit of tryptophan synthase for production of non-canonical amino acids
AssigneeCalifornia Institute of Technology
InventorsAndrew Richard Buller, Sabine Brinkmann-Chen, Michael Herger, David K. Romney, Javier Murciano Calles, Paul van Roye
Life of this patent
Estimating the term…
Abstract
This disclosure relates to modified tryptophan synthase and more particularly to modified beta-subunits of tryptophan synthase. The disclosure further relates to cells expressing such modified subunits and methods of producing non-canonical amino acids.
Claims30
A recombinant polypeptide selected from the group consisting of (a) and (b) as follows:
- (a) a polypeptide comprising a sequence that is one of SEQ ID NOS:
- 2, 4, 6, or 8 containing up to 50 amino acid substitutions,
- wherein the polypeptide comprises a mutation at positions corresponding to residue 144 and 166 of SEQ ID NO:2, and
- wherein the polypeptide can catalyze the production of tryptophan or a non-canonical amino acid or unnatural amino acid from an indole or indole derivative and L-serine or L-threonine independent of any other tryptophan synthase subunit;
- and
- (b) a polypeptide comprising a sequence that is one of SEQ ID NOS:
- 2, 4, or 6 containing up to 50 amino acid substitutions,
- wherein the polypeptide comprises a mutation at the position corresponding to residue 292 of SEQ ID NO:2, wherein the residue corresponding to residue 292 is mutated to a Serine (S), and
- wherein the polypeptide can catalyze the production of tryptophan or a non-canonical amino acid or unnatural amino acid from an indole or indole derivative and L-serine or L-threonine independent of any other tryptophan synthase subunit.
6 more elements
Dependencies are inferred from the claim text.