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US 10,513,691 B2Granted
Methods for printing organs and organoids
AssigneePrellis Biologics, Inc.
InventorsMelanie P. Matheu, Kathryn J. Parkinson, Emma R. Moulton
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Abstract
Provided herein are methods and systems for bio-printing of three-dimensional organs and organoids. Also provided herein are bio-printed three-dimensional organs and organoids for use in the generation and/or the assessment of immunological products and/or immune responses. Also provided herein are methods and system for bio-printing three-dimensional matrices.
Claims29
A method for printing an organoid, comprising:
- (a) providing a media chamber comprising a medium comprising (i) a plurality of cells and (ii) one or more polymer precursors;
- and
- (b) using at least one energy beam to generate a patterned three-dimensional (3D) holographic projection in said medium in said media chamber along at least one energy beam path that is patterned into a three-dimensional (3D) projection in accordance with computer instructions for printing a 3D organoid in computer memory, to form at least a portion of said 3D organoid comprising (i) at least a subset of said plurality of cells, and (ii) a polymer formed from said one or more polymer precursors.
A method for printing an organoid, comprising:
- (a) providing a media chamber comprising a first medium, wherein said first medium comprises a first plurality of cells and a first polymeric precursor;
- (b) using at least one energy beam to generate a patterned three-dimensional (3D) holographic projection in said first medium in said media chamber along at least one energy beam path in accordance with computer instructions for printing a three-dimensional (3D) organoid in computer memory, to subject at least a portion of said first medium in said media chamber to form a first portion of said 3D organoid;
- (c) providing a second medium in said media chamber, wherein said second medium comprises a second plurality of cells and a second polymeric precursor, wherein said second plurality of cells is of a different type than said first plurality of cells;
- and
- (d) using at least one energy beam to generate a patterned three-dimensional (3D) holographic projection in said second medium in said media chamber along at least one energy beam path in accordance with said computer instructions, to subject at least a portion of said second medium in said media chamber to form a second portion of said 3D organoid.
2 more elements
Dependencies are inferred from the claim text.