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US 10,512,910 B2Granted
Droplet-based analysis method
AssigneeBio-Rad Laboratories, Inc.
InventorsBilly W. Colston, Jr., Benjamin J. Hindson, Kevin D. Ness, Donald A. Masquelier
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Abstract
Droplet-based methods of analysis. In an exemplary method, a device having a port connected to a chamber may be selected. A sample-containing fluid may be placed into the port. A pressure differential may be created that drives the sample-containing fluid from the port to the chamber and separates the sample-containing fluid into droplets. A two-dimensional monolayer of the droplets may be formed in the chamber. At least a portion of the monolayer may be imaged.
Claims20
A method of analysis, the method comprising:
- selecting a device having a port connected to a chamber;
- placing a sample-containing fluid into the port;
- creating a pressure differential that drives the sample-containing fluid from the port to the chamber and separates the sample-containing fluid into droplets;
- forming a two-dimensional monolayer of the droplets in the chamber;
- and
- imaging at least a portion of the monolayer.
3 more elements
A method of analysis, the method comprising:
- selecting a device having a chamber connected separately to a port and a vent;
- placing an aqueous fluid into the port;
- applying gas pressure to the device to drive the aqueous fluid from the port to the chamber and form partitions of the aqueous fluid, the partitions being separated from one another by a carrier liquid;
- amplifying a nucleic acid target in only of a subset of the partitions while the partitions are arranged in a two-dimensional monolayer in the chamber;
- imaging at least a portion of the monolayer to create one or more images;
- and
- determining whether individual partitions of the monolayer contain the nucleic acid target using the one or more images.
4 more elements
Dependencies are inferred from the claim text.