Pseudo HandE image producing method and optical system using same

An optical system comprising:

• a first photo sensing unit, a second photo sensing unit, an information processing apparatus, and a display unit, characterized in that:
• the first photo sensing unit is used for inputting a grayscale interference image or a grayscale reflected image of a pathological sample to a first memory block of the information processing apparatus, the grayscale interference image or the grayscale reflected image having a first image resolution, and the second photo sensing unit is used for inputting a grayscale fluorescence image of the pathological sample to a second memory block of the information processing apparatus, the grayscale fluorescence image having a second image resolution, and the first image resolution being same with or different from the second image resolution;
• the information processing apparatus has a first color transform operation unit coupled with the first memory block for performing a first color transform operation on the grayscale interference image or the grayscale reflected image to generate a first RGB image, and has a second color transform operation unit coupled with the second memory block for performing a second color transform operation on the grayscale fluorescence image to generate a second RGB image;
• the information processing apparatus has an image fusion operation and intensity reversal operation unit for performing an image fusion operation and an intensity reversal operation on the first RGB image and the second RGB image to generate a pseudo H&E image;
• and the information processing apparatus outputs the pseudo H&E image to the display unit;
• wherein the first RGB image is a black-background dark green image;
• the second RGB image is a black-background yellow-green image;
• and the pseudo H&E image is a white-background pink-and-blue-violet image.