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US 10,508,295 B2Granted
Gamma glutamyl-valine synthase, and method for producing gamma glutamyl-valyl-glycine
AssigneeAjinomoto Co., Inc.
InventorsChihiro Tsuji, Hiroyuki Nozaki
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Abstract
γ-Glu-Val synthetase suitable for generating γ-Glu-Val, and a method for producing γ-Glu-Val-Gly using the same are provided. By using γ-Glu-Val synthetase showing a ratio of γ-glutamylvaline synthetase activity to γ-glutamylglycine synthetase activity of 2.0 or higher, such as γ-Glu-Val synthetase of Kocuria rosea (AJ3132), γ-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.
Claims19
A method for producing γ-Glu-Val-Gly and/or a salt thereof, the method comprising:
- contacting a protein and glutathione synthetase with Glu, Val, and Gly to produce γ-Glu-Val-Gly;
- and
- collecting the produced γ-Glu-Val-Gly,
- wherein the protein comprises an amino acid sequence having at least 97% sequence identity to the amino acid sequence of SEQ ID NO:
- 6, 10, or 16, and has γ-glutamylvaline synthetase activity, and
- a ratio of γ-glutamylvaline synthetase activity of the protein to γ-glutamylglycine synthetase activity of the protein is 15 or higher.
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