Matrix in ball form as a cell carrier
AssigneeMaco Pharma
InventorsThomas Bouckenooghe, Pauline Bertholet, Bruno Delorme
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Abstract
The invention relates to a matrix in ball form comprising cross-linked fibrinogen, the matrix being free from fibrin, as well as to a method for preparing such a matrix, comprising the following steps: (a) providing an initial composition comprising fibrinogen and a platelet factor; (b) injecting said initial composition into an oil heated to a temperature of 50° C. to 80° C. so as to form an emulsion; (c) mixing the emulsion thus obtained at a temperature of 50° C. to 80° C. until a matrix in ball form is obtained; and (d) isolating the matrix thus obtained. The matrix is used as a cell carrier.
Claims17
A method for preparing a matrix comprising the following steps:
- (a) providing an initial composition comprising fibrinogen and a platelet factor the initial composition being free of exogenous thrombin;
- (b) injecting said initial composition in the absence of exogenous thrombin into an oil heated to a temperature of 50° C. to 80° C. so as to form an emulsion;
- (c) mixing the emulsion thus obtained at a temperature of 50° C. to 80° C. until a matrix in ball form is obtained;
- and
- (d) isolating the matrix thus obtained.
A method for preparing a fibrin-free, cross-linked fibrinogen matrix, comprising the following steps:
- (a) providing is a platelet lysate free of exogenous thrombin and obtained by lysis of platelets in suspension in plasma;
- (b) injecting said platelet lysate into an oil heated to a temperature of 50° C. to 80° C. so as to form an emulsion;
- (c) mixing the emulsion thus obtained at a temperature of 50° C. to 80° C. until a matrix in ball form is obtained;
- and
- (d) isolating the matrix thus obtained.
Dependencies are inferred from the claim text.