Altering microbial populations and modifying microbiota
AssigneeSNIPR Technologies Limited
InventorJasper Clube
Life of this patent
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Abstract
The invention relates to methods, uses, systems, arrays, engineered nucleotide sequences and vectors for inhibiting bacterial population growth or for altering the relative ratio of sub-populations of first and second bacteria in a mixed population of bacteria. The invention is particularly useful, for example, for treatment of microbes such as for environmental, medical, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system.
Claims23
A method for treating an infection caused by bacterial or archaeal host cells in an organism, the method comprising:
- (a) contacting the host cells with an engineered nucleic acid for producing a host modifying crRNA (HM-crRNA), and
- (b) producing the HM-crRNA in the host cells;
- wherein
- (i) the HM-crRNA is operable with a Type I Cas in the host cells, wherein the engineered nucleic acid and the Type I Cas are comprised by a Type I HM-CRISPR/Cas system in the host cells;
- (ii) the HM-crRNA comprises a nucleotide sequence that is capable of hybridizing to the target sequence in the host cells to guide the Type I Cas to modify the target sequence in the host cells;
- and
- (iii) wherein the Type I Cas is an endogenous Cas in the host cells;
- wherein the target sequence is modified by the Type I HM-CRISPR/Cas system and the infection is treated.
Dependencies are inferred from the claim text.