# US 10,504,713 B2 · Frequency scan linear ion trap mass spectrometry

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- Number: US 10,504,713 B2
- Document ID: US10504713B2
- Status: Granted
- Kind: B2
- Application number: 15/046,387
- Filed: 2016-02-17
- Published: 2016-06-09
- Granted: 2019-12-10
- Earliest claimed: 2011-06-28
- Assignees: Academia Sinica
- Applicants: Academia Sinica
- Inventors: Chung-Hsuan Chen; I-Chung Lu; Ming-Lee Chu
- Web page: https://patentgrep.com/patent/US10504713B2

## CPC

- H01J 49/429: {Scanning an electric parameter, e.g. voltage amplitude or frequency}
- H01J 49/161: {using photoionisation, e.g. by laser}
- H01J 49/4225: {Multipole linear ion traps, e.g. quadrupoles, hexapoles}
- H01J 49/022: {Circuit arrangements, e.g. for generating deviation currents or voltages (regulating electric or magnetic variables in general, e.g. current, magnetic field G05F); Components associated with high voltage supply (high voltage supply per se H02M)}
- H01J 49/164: {Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI] (sample holders H01J49/0418)}
- H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H)

## Abstract

An ion trap mass spectrometer and methods for obtaining a mass spectrum of ions by scanning an RF frequency applied to the linear ion trap for mass selective ejection of the ions by using two power amplifiers to apply opposite phases of the RF to x and y electrodes.

## Claims

### Claim 1

A method for obtaining a mass spectrum of biomolecular ions, the method comprising:
providing a two dimensional linear ion trap comprising x and y electrodes, wherein the x and y electrodes are consisting of two x electrode rods and two y electrode rods in a quadrupole arrangement, wherein the two dimensional linear ion trap is non-segmented and is the only ion trap used, and wherein the linear ion trap comprises two end plates perpendicular to the rods to confine or release ions in the z axial direction with an axial trapping field;
scanning an RF frequency stepwise, with fixed amplitude applied to the linear ion trap for ion trapping and mass selective ejection of the ions, wherein the phase of the scanning RF and trapping RF are matched, wherein the RF phases are also matched to the timing of laser shots which generate the ions, and wherein the two x and two y quadrupole rods are synchronized using two power amplifiers to apply opposite phases of the RF to the x and y electrodes, wherein each power amplifier is tuned with a capacitance to provide the same amplitude of RF as the other amplifier and a fixed degree of phase difference of the RF to the x and y electrodes during the RF frequency scan;
detecting the biomolecular ions ejected from the linear ion trap using a charge detector, wherein the ions have an m/z of at least 150,000 Da/q.

### Claim 2 (depends on 1)

The method of claim 1, wherein the mass selective ejection of the ions is generated by mass selective instability with or without resonance excitation by boundary ejection.

### Claim 3 (depends on 1)

The method of claim 1, wherein the ejection of the ions is axial or perpendicular.

### Claim 4 (depends on 1)

The method of claim 1, wherein the ejection of the ions is through a slot in an electrode.

### Claim 5 (depends on 1)

The method of claim 1, wherein the linear ion trap has end plate electrodes that are perpendicular to the rods and spaced apart from each end of the rods, and wherein a fixed voltage of from +5 to +200 V is applied to the end plates.

### Claim 6 (depends on 1)

The method of claim 1, wherein the linear ion trap contains a buffer gas of helium or other rare gas at a pressure of from 1 to 500 mTorr.

### Claim 7 (depends on 1)

The method of claim 1, wherein the ions are generated by MALDI, electrospray ionization, laser ionization, thermospray ionization, thermal ionization, electron ionization, chemical ionization, inductively coupled plasma ionization, glow discharge ionization, field desorption ionization, fast atom bombardment ionization, spark ionization, or ion attachment ionization.

### Claim 8

A linear ion trap mass spectrometer for obtaining a mass spectrum of biomolecular ions, the linear ion trap mass spectrometer comprising:
a single, non-segmented two dimensional linear ion trap for trapping and ejecting the ions having x and y electrodes consisting of two slotted x electrode rods and two y electrode rods in a quadrupole arrangement, and wherein the two dimensional linear ion trap is the only ion trap used;
two power amplifiers to apply opposite phases of trapping RF to the x and y electrodes, wherein the two power amplifiers synchronize the two x and two y quadrupole rods, wherein each power amplifier is tuned with a capacitance to provide the same amplitude of RF as the other amplifier and a fixed degree of phase difference of the RF to the x and y electrodes during a stepwise, RF frequency scan with fixed amplitude; wherein the phase of the scanning RF and trapping RF are matched, wherein the RF phases are also matched to the timing of laser shots which generate the ions, and wherein the ions have an m/z of at least 150,000 Da/q;
an inductance forming an LC circuit with the capacitance of the ion trap;
a first end cap plate perpendicular to the electrode rods at a first end of the linear ion trap and a second end cap plate perpendicular to the electrode rods at a second end of the linear ion trap, wherein the first end cap defines an opening for a sample probe, and wherein the second end cap defines an opening for exit of ions and for passing a laser beam, wherein the end plates confine or release ions in the z axial direction with an axial trapping field.

### Claim 9 (depends on 8)

The linear ion trap mass spectrometer of claim 8, wherein the end plates are spaced apart by 1 to 10 mm from the ends of the electrode rods.

### Claim 10 (depends on 8)

The linear ion trap mass spectrometer of claim 8, further comprising a buffer gas within the linear ion trap.

### Claim 11 (depends on 10)

The linear ion trap mass spectrometer of claim 10, wherein the buffer gas is helium or other rare gas at a pressure of from 1 to 500 mTorr.

### Claim 12 (depends on 8)

The ion trap mass spectrometer of claim 8, wherein the ions are generated by MALDI, electrospray ionization, laser ionization, thermospray ionization, thermal ionization, electron ionization, chemical ionization, inductively coupled plasma ionization, glow discharge ionization, field desorption ionization, fast atom bombardment ionization, spark ionization, or ion attachment ionization.