# US 10,502,942 B2 · Integrated illuminator and condenser for microscopes

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- Number: US 10,502,942 B2
- Document ID: US10502942B2
- Status: Granted
- Kind: B2
- Application number: 15/178,672
- Filed: 2016-06-10
- Published: 2016-12-15
- Granted: 2019-12-10
- Earliest claimed: 2015-06-12
- Assignees: Techshot, Inc.
- Applicants: Techshot, Inc.
- Inventors: Paul W. Todd; N. Samuel Logan; Michael A. Kurk; Nathan A. Thomas; Paul Faget
- Web page: https://patentgrep.com/patent/US10502942B2

## CPC

- G02B 21/125: {affording both dark- and bright-field illumination}
- F21V 23/003: {the elements being electronics drivers or controllers for operating the light source, e.g. for a LED array}
- G02B 21/02: Objectives
- G02B 21/06: Means for illuminating specimens
- G02B 21/08: Condensers
- G02B 21/086: {for transillumination only}
- G02B 21/14: affording illumination for phase-contrast observation
- G02B 21/16: adapted for ultraviolet illumination {; Fluorescence microscopes (G02B21/0076 takes precedence)}
- G02B 27/30: Collimators
- G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- F21V: FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR

## Abstract

A Light-Emitting Diode (LED) illuminator-condenser capable of supplying bright-field, dark-field, fluorescence, oblique, and phase-contrast illumination under Köhler-like conditions. A single lens may be permanently focused on the specimen plane. Illumination conditions may be made by a combination of uniform LEDs, collimating masks and diffusers. LEDs may be arranged in one or more ring-shaped patterns.

## Claims

### Claim 1

An integrated illuminator and condenser for microscopes, in which illuminator and condenser functions are coupled, comprising:
a single lens wherein the number of lenses with refractive power in the integrated illuminator and condenser is equal to one, and wherein said single lens is a convergent lens;
at least one multiwavelength light-emitting diode for bright field illumination passing through said single lens;
a first circular array of green light-emitting diodes for low-magnification phase contrast illumination passing through said single lens;
at least one second circular array of green light-emitting diodes for high-magnification phase contrast illumination passing through said single lens, said at least one second circular array of green light-emitting diodes for high-magnification phase contrast illumination having a diameter greater than said first circular array of green light-emitting diodes for low-magnification phase contrast illumination;
a third circular array having at least one of multiwavelength light-emitting diodes for dark field illumination, single-wavelength light-emitting diodes for fluorescence illumination, or a combination thereof, and wherein the dark field illumination, the fluorescence illumination, or the combination thereof from said third circular array does not pass through any lens element with refractive power; and
one or more light-absorbing annular masks having a fixed aperture geometry to limit light from one or more of said circular arrays of light-emitting diodes.

### Claim 2 (depends on 1)

An illuminated microscope comprising
a microscope having 10× and 20× phase-contrast microscope objective lenses;
and the integrated illuminator and condenser of claim 1 wherein said first circular array of green light-emitting diodes is of a diameter for low-magnification phase contrast illumination for 10× and 20× magnification.

### Claim 3 (depends on 1, 2)

The illuminated microscope of claim 2 further comprising 40× or higher magnification phase-contrast microscope objective lenses;
and the integrated illuminator and condenser of claim 1 wherein said diameter of said at least one second circular array of green light-emitting diodes for high-magnification phase contrast illumination is for 40× or higher magnification.

### Claim 4 (depends on 1)

The integrated illuminator and condenser according to claim 1 in which said third circular array includes multiwavelength light-emitting diodes for dark-field illumination of a diameter and includes individual light-emitting diodes so directed as to illuminate specimens with light that is obliquely incident at the specimen plane.

### Claim 5 (depends on 1)

The integrated illuminator and condenser according to claim 1 in which said third circular array includes single-wavelength light-emitting diodes for fluorescence illumination of a diameter and includes individual light-emitting diodes so directed as to illuminate specimens with light that is obliquely incident at the specimen plane.

### Claim 6 (depends on 1)

The integrated illuminator and condenser according to claim 1 in which said third circular array includes single-wavelength light-emitting diodes for fluorescence illumination emitting light having a wavelength that is capable of exciting fluorescence.

### Claim 7 (depends on 1)

The integrated illuminator and condenser according to claim 1 in which said one or more light-absorbing annular masks form rings of light that define parallel-propagating light incident on said single lens emitted from said first circular array of green light-emitting diodes and said at least one second circular array of green light-emitting diodes.

### Claim 8 (depends on 1)

The integrated illuminator and condenser according to claim 1 further including electrical switches, wherein said electrical switches activate said at least one multiwavelength light-emitting diode, said first circular array of green light-emitting diodes, said at least one second circular array of green light-emitting diodes, and said third circular array.

### Claim 9 (depends on 1)

The integrated illuminator and condenser according to claim 1 further including a computer, wherein said computer activates said at least one multiwavelength light-emitting diode, said first circular array of green light-emitting diodes, said at least one second circular array of green light-emitting diodes, and said third circular array said computer being connected by cable or by wireless means.

### Claim 10 (depends on 9)

The integrated illuminator and condenser according to claim 9 in which said computer is operated by screen commands using a graphical user interface.

### Claim 11 (depends on 1)

The integrated illuminator and condenser according to claim 1 wherein each one of said at least one multiwavelength light-emitting diode, said first circular array of green light-emitting diodes, and said at least one second circular array of green light-emitting diodes is a planar LED source.

### Claim 12 (depends on 1)

The integrated illuminator and condenser according to claim 1 wherein said one or more light-absorbing annular masks limit at least one of an inner diameter and an outer diameter of the light from one or more of said circular arrays of light-emitting diodes.

### Claim 13 (depends on 1)

The integrated illuminator and condenser according to claim 1 further comprising a thin layer of light-diffusing material.

### Claim 14 (depends on 1)

The integrated illuminator and condenser according to claim 1 wherein said one or more light-absorbing annular masks limits light from each one of said circular arrays of light-emitting diodes.

### Claim 15 (depends on 1)

The integrated illuminator and condenser according to claim 1 wherein one or more of said light-emitting diodes are an organic-LED (OLED) display plane.

### Claim 16 (depends on 15)

The integrated illuminator and condenser according to claim 15 further including a computer, wherein said computer controls said OLED display plane to produce at least one of bright-light, phase contrast, dark-field and fluorescence illumination.

### Claim 17 (depends on 15)

The integrated illuminator and condenser according to claim 15 further including a computer, wherein said computer controls said OLED display plane to produce light-source images having various shapes.

### Claim 18 (depends on 15)

The integrated illuminator and condenser according to claim 15 further including a computer, wherein said computer controls said OLED display plane to produce light-source images having various shapes suitable for bright field, dark field, phase contrast and fluorescence excitation illumination.

### Claim 19 (depends on 15)

The integrated illuminator and condenser according to claim 15 further including a computer, wherein said computer controls said OLED display plane to produce light-source images having various spectra.

### Claim 20 (depends on 19)

The integrated illuminator and condenser according to claim 19 in which said OLED display plane is controlled by said computer to produce light-source images having various spectra suitable for bright field, dark field, phase contrast and fluorescence excitation illumination.

### Claim 21 (depends on 1)

An illuminated microscope comprising
a microscope including a condenser carrier;
and the integrated illuminator and condenser of claim 1 further including a mounting cylinder and a circuit board, wherein said mounting cylinder is provided below said circuit board for the purpose of mounting said integrated illuminator and condenser to said condenser carrier of said microscope.

### Claim 22 (depends on 1)

An illuminated microscope comprising
a microscope including a condenser carrier;
and the integrated illuminator and condenser of claim 1 further including a mounting cylinder and a circuit board, wherein said mounting cylinder is provided above said circuit board for the purpose of mounting said integrated illuminator and condenser to said condenser carrier of said microscope.

### Claim 23 (depends on 1)

An illuminated microscope comprising
a microscope including a microscope body and a specimen plane;
and the integrated illuminator and condenser of claim 1.

### Claim 24 (depends on 1)

The integrated illuminator and condenser according to claim 1 further comprising at least one circular array of a plurality of said at least one multiwavelength light-emitting diodes for bright field illumination.

### Claim 25 (depends on 1)

The integrated illuminator and condenser according to claim 1 wherein said single lens, said at least one multiwavelength light-emitting diode, said first circular array, said second circular array, and said third circular array are fixed relative to each other.
