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US 10,502,750 B2Granted
Reliable and robust method for the analysis of cannabinoids and terpenes in cannabis
AssigneeBiotech Institute, LLC
InventorsMatthew W. Giese, Mark Anthony Lewis, Laura Giese
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Abstract
The present invention teaches methods useful for accurately and precisely analyzing cannabis plants, plant parts, and extract samples. In particular, the present invention teaches methods of extracting, and quantifying cannabinoid and terpene constituents.
Claims20
A method for determining a concentration of at least one cannabinoid and at least one terpene in a test sample, the method comprising the steps of:
- a) extracting the test sample in an extraction solution, thereby producing an extracted test sample comprising the at least one cannabinoid and at least one terpene dissolved in the extraction solution, wherein the extraction solution comprises:
- i) a solvent;
- ii) a first internal standard with a known response ratio relative to the at least one cannabinoid;
- iii) a second internal standard with a known response ratio relative to the at least one terpene;
- and
- iv) a third internal standard;
- b) diluting the extracted test sample produced by step a) one or more times with a dilution solution;
- wherein said dilution solution comprises the solvent, the first internal standard, and the second internal standard, but not the third internal standard;
- c) subjecting the extracted test sample produced by step a), and/or the diluted test sample(s) produced by step b) to at least one chromatographic separation and detecting the separated sample(s) with at least one detector;
- and
- d) determining the concentration of the at least one cannabinoid and the at least one terpene in the test sample;
- wherein the concentration of the at least one cannabinoid in the test sample is normalized for dilution errors based on the concentration of the third internal standard in the diluted test sample(s) produced by step b).
9 more elements
A method for quantifying cannabinoids and terpenes from a test sample, said method comprising the steps of:
- a) extracting the test sample in an extraction solution, thereby producing an analytical extract comprising the cannabinoids and terpenes dissolved in the extraction solution, said extraction solution comprising:
- i. a solvent;
- ii. a first internal standard, and;
- iii. a second internal standard;
- and
- b) performing a high performance liquid chromatography (HPLC), and a gas chromatography-flame ionization detector (GC-FID) analysis of the analytical extract of step a) to produce a signal for at least one cannabinoid and at least one terpene, and to produce a signal for each of the standards;
- wherein the signal for the at least one cannabinoid is normalized based on the signal from the first internal standard to quantify said at least one cannabinoid in the test sample, and wherein the signal for the at least one terpene is normalized based on the signal from the second internal standard to quantify said at least one terpene in the test sample;
- wherein the first internal standard is ibuprofen at a known concentration, and the second internal standard is n-nonane at a known concentration.
5 more elements
Dependencies are inferred from the claim text.