# US 10,502,749 B1 · Method for patient stratification and drug efficacy monitoring

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- Number: US 10,502,749 B1
- Document ID: US10502749B1
- Status: Granted
- Kind: B1
- Application number: 15/617,820
- Filed: 2017-06-08
- Granted: 2019-12-10
- Earliest claimed: 2016-06-08
- Assignees: New York University
- Applicants: New York University
- Inventors: Heather L. Bowling; Aditi Bhattacharya; Eric Klann
- Web page: https://patentgrep.com/patent/US10502749B1

## CPC

- G01N 33/6893: {related to diseases not provided for elsewhere}
- A61K 31/506: not condensed and containing further heterocyclic rings
- C07K 16/40: against enzymes
- C12Q 1/34: involving hydrolase
- C12Y 306/05002: Small monomeric GTPase (3.6.5.2)
- G01N 33/573: for enzymes or isoenzymes
- G01N 2333/914: Hydrolases (3)
- G01N 2800/52: Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
- G01N 2800/60: Complex ways of combining multiple protein biomarkers for diagnosis
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Y: ENZYMES

## Abstract

Identified are markers whose de novo synthesis or levels in blood provide are correlative information on the status of pathology, regardless of whether or not they correlate with the status in the relevant organ. The de novo synthesis or levels in blood can be used for patient stratification and monitoring the efficacy of treatment of diseases, such as fragile X syndrome.

## Claims

### Claim 1

A panel consisting of antibodies for the detection of protein markers metabotropic glutamate receptor 5 (mGLUR5), glutamate ionotropic receptor NMDA type subunit 2B (Grin2B), Ras, hexokinase, aconitase 2 (ACO2), eukaryotic translation initiation factor 2a (eIF2a), serine-threonine protein kinase (PAK), synaptic Ras GTPase-activating protein 1 (Syngap), and acyl-CoA-binding protein (DBI), which markers are indicative of the status of fragile X syndrome, each said antibody being specific for mGLUR5, Grin2B, Ras, hexokinase, ACO2, eIF2a, PAK, Syngap, or DBI.

### Claim 2

A panel consisting of antibodies for the detection of protein markers aconitase 2 (ACO2), Ras, hexokinase, and metabotropic glutamate receptor 5 (mGLUR5) and glutamate ionotropic receptor NMDA type subunit 2B (Grin2B), which markers are indicative of the status of fragile X syndrome, each said antibody being specific for ACO2, Ras, hexokinase, Grin2B, or mGLUR5.

### Claim 3

A panel consisting of antibodies for the detection of protein markers aconitase 2 (ACO2), Ras, and hexokinase, which markers are indicative of the status of fragile X syndrome, each said antibody being specific for ACO2, Ras, or hexokinase.

### Claim 4

A panel of antibodies consisting of antibodies for the detection of protein markers: i) aconitase 2 (ACO2), ii) Ras, iii) hexokinase, and iv) metabotropic glutamate receptor 5 (mGLUR5) or glutamate ionotropic receptor NMDA type subunit 2B (Grin2B); which markers are indicative of the status of fragile X syndrome, each said antibody being specific for ACO2, Ras, hexokinase, mGLUR5 or Grin2B.