# US 10,502,734 B2 · System for biodetection applications > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,502,734 B2 - Document ID: US10502734B2 - Status: Granted - Kind: B2 - Application number: 15/315,029 - Filed: 2015-06-02 - Published: 2017-07-20 - Granted: 2019-12-10 - Earliest claimed: 2014-06-03 - Assignees: Consejo Superior de Investigaciones Cientificas - Applicants: Consejo Superior De Investigaciones Cientificas - Inventors: Francisco Javier Tamayo de Miguel; Priscila Monteiro Kosaka; Valerio Pini; Montserrat Calleja Goméz; Jose Jaime Ruz Martinez; Daniel Ramos Vega; Maria Ujue Gonzalez Sagardoy - Web page: https://patentgrep.com/patent/US10502734B2 ## CPC - G01N 33/54346: {Nanoparticles} - G01N 21/47: Scattering, i.e. diffuse reflection (G01N21/25, G01N21/41 take precedence {G01N21/55 takes precedence}) - G01N 21/554: {detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance} - G01N 21/59: Transmissivity (G01N21/25 takes precedence) - G01N 21/658: {enhancement Raman, e.g. surface plasmons} - G01N 21/77: by observing the effect on a chemical indicator - G01N 21/7703: {using reagent-clad optical fibres or optical waveguides (using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B)} - G01N 33/54373: {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings} - G01N 2021/5903: {using surface plasmon resonance [SPR], e.g. extraordinary optical transmission [EOT]} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The present invention relates to a system for biodetection applications comprising two basic elements, a substrate with a functionalized surface and a nanoparticle, the system being capable of enhancing the plasmonic effect of the nanoparticle. The invention also relates to a biosensor incorporating such system, in addition to the method for detecting and quantifying a target analyte selected in a sample using such system. Finally, the invention relates to a device which can detect the enhanced optoplasmonic effect of the nanoparticles by means of the system of the invention or by combining the detection of such optoplasmonic effect with the analysis of the changes in the mechanical characteristics in the substrate. ## Claims ### Claim 1 A system for biodetection applications comprising: a. a substrate of dielectric material having at least one surface functionalized with a recognition element which can bind specifically to a target analyte, the dielectric material being surrounded by a surrounding material wherein the substrate acts as a mechanical resonator and optical cavity; and b. at least one nanoparticle with plasmonic properties which comprises at least one detection element bound thereto and which can bind specifically to the target analyte in a sandwich-type arrangement, characterized in that: the substrate of dielectric material has a thickness between 0.1 μm and 5 μm and an extinction coefficient less than 0.3, the nanoparticle has at least one of its dimensions with a size of 2 nm to 300 nm, the ratio between the refractive index of the dielectric material and the surrounding material is greater than 1.1, and in that the system detection is performed by an optoplasmonic detection of an enhanced plasmonic effect. ### Claim 2 (depends on 1) The system according to claim 1, wherein the dielectric material is selected from quartz, silicon, silicon nitride, silicon carbide, graphene, polymers and hydrogels. ### Claim 3 (depends on 1) The system according to claim 1, wherein the substrate is in the form of a microcantilever, a micropillar, a string resonator, a trampoline resonator, a rectangular cantilever, a triangular cantilever, a pyramidal cantilever, a blade cantilever, a membrane resonator, a plate resonator, a bridge, a hollow cantilever or a nanowire. ### Claim 4 (depends on 1) The system according to claim 1, wherein the substrate is functionalized with a recognition element selected from an antibody, a receptor, a peptide, a protein, a carbohydrate, a nucleic acid, a cell, a microorganism or a part thereof. ### Claim 5 (depends on 1) The system according to claim 1, wherein the detection element is selected from an antibody or a nucleic acid molecule. ### Claim 6 (depends on 1) The system according to claim 1, wherein the nanoparticle is a gold nanoparticle, a silver nanoparticle or a nanoparticle of plasmonic metamaterial. ### Claim 7 (depends on 1) The system according to claim 1, wherein the nanoparticle has a structure selected from the group of nanospheres, nanorods, pointed nanorods, nanoshells, nanocages/frames, hollow nanospheres, tetrahedra, octahedra, cubes, icosahedra, rhombic dodecahedra, concave nanocubes, tetrahexahedra, obtuse triangular bipyramids, trisohectahedra and nanoprisms. ### Claim 8 (depends on 1) The system according to claim 1, wherein the substrate has a transmittance index comprised between 0.01 and 1 and/or a reflectance index comprised between 0.01 and 1. ### Claim 9 (depends on 1) A biosensor comprising the system according to claim 1. ### Claim 10 (depends on 1) A biosensor arranged in the form of an array comprising multiple systems according to claim 1, each system comprising a substrate designed for detecting a different target analyte or different concentrations of the same analyte. ### Claim 11 (depends on 1) A device for surface inspection arranged for detecting an optoplasmonic effect in at least one nanoparticle, said device comprising: the system according to claim 1, an electromagnetic radiation source arranged for generating at least one electromagnetic radiation beam; a first sensitive detector arranged for receiving the electromagnetic radiation when it is reflected from or transmitted through the substrate for producing at least a first output signal in response to the scattering and/or extinction of said electromagnetic radiation; and an electronic control system. ### Claim 12 (depends on 11) The device according to claim 11, wherein the device further comprises: a subsystem for detecting a change in a mechanical characteristic in the substrate, said subsystem comprising a second sensitive detector arranged for detecting a mechanical change in the substrate for producing at least a second signal in response to said mechanical change; and means for producing a final output signal based on the combination of first and second output signals of the first and second sensitive detectors. ### Claim 13 (depends on 12) The device according to claim 12, wherein said detection subsystem comprises: an illumination light or laser beam and a linear position sensitive photodetector for recording the change in the mechanical characteristic on the substrate; an electronic control system; scanning means for scanning the relative shift of said light or laser beam with respect to the substrate such that they scan the substrate with the light beam following instructions from the electronic control system.