# US 10,502,668 B2 · Method of collecting target cells

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- Number: US 10,502,668 B2
- Document ID: US10502668B2
- Status: Granted
- Kind: B2
- Application number: 15/150,311
- Filed: 2016-05-09
- Published: 2016-09-01
- Granted: 2019-12-10
- Earliest claimed: 2010-10-25
- Assignees: Cytogen Corporation
- Applicants: Cytogen Co., Ltd.
- Inventors: Byung Hee Jeon; Jong Kil Lee
- Web page: https://patentgrep.com/patent/US10502668B2

## CPC

- G01N 1/4077: {by other techniques involving separation of suspended solids}
- C12M 47/02: {Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02)}
- C12M 47/04: {Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04)}
- G01N 1/34: Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)}
- B01L 3/50255: {Multi-well filtration}
- G01N 2001/4088: {filtration}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

A method of collecting target cells including providing a cell collection device comprising a conduit defining a fluid channel, a first screen and a second screen, the first and second screens are movable relative to each other along the flow direction between a first configuration and a second configuration, flowing a fluid composition comprising the target cells in the flow direction while the first and second screens are in a first configuration to screen at least part of the target cells, moving the first screen relative to the second screen along the flow direction from the first configuration to form the second configuration, to permit at least part of the target cells screened in the first configuration to pass the first screen first and then the second screen, and collecting at least part of the target cells at the second end of the fluid channel.

## Claims

### Claim 1

A method of collecting target cells, the method comprising:
providing a cell collection device comprising a conduit defining a fluid channel, a first screen defining pores and a second screen defining second pores, the pores of the first screen comprising a first pore, the pores of the second screen comprising a second pore, the first and second screens being arranged in the fluid channel such that liquid flowing through the fluid channel in a flow direction passes the first screen first and the second screen second,
wherein the first and second screens are movable relative to each other along the flow direction between a first configuration and a second configuration of the cell collection device, wherein in the first configuration, the first and second screens are at a first distance therebetween in the flow direction whereas in the second configuration, the first and second screens are at a second distance therebetween in the flow direction that is larger than the first distance,
wherein in the first configuration, the first and second screens in combination form a combined filter, in which the first pore of the first screen and the second pore of the second screen are partially overlapping when viewed in the flow direction such that the combined filter is configured to screen target cells that would pass the first screen and the second screen individually, whereas in the second configuration, each of the first and second screens is to act independently;
flowing a fluid composition comprising the target cells from a first end of the fluid channel toward a second end of the fluid channel in the flow direction while the cell collection device is in the first configuration, and screening at least part of the target cells by the combined filter and allowing at least part of the rest of the fluid composition to pass through the combined filter toward the second end of the fluid channel;
subsequently, moving the first screen relative to the second screen along the flow direction from the first configuration to form the second configuration, to permit at least part of the target cells screened by the combined filter in the first configuration to pass the first screen first and then the second screen in the flow direction; and
collecting at least part of the target cells at the second end of the fluid channel,
wherein the conduit comprises a first tube and a second tube that are arranged along the flow direction, wherein the first screen is attached to the first tube, and the second screen is attached to the second tube.

### Claim 2 (depends on 1)

The method of claim 1, wherein the first screen is attached to an end of the first tube, wherein the end of the first tube and the first screen are inserted into the second tube and configured to move relative to the second tube along the flow direction.

### Claim 3 (depends on 1)

The method of claim 1, wherein each of the first and second tubes comprises a threaded wall, wherein the threaded wall of the first tube is engaged with the threaded wall of the second tube such that the first and second tubes can move relative to each other in the flow direction.

### Claim 4 (depends on 1)

The method of claim 1, wherein at least part of the pores of the first screen is in a rectangular shape, wherein at least part of the pores of the second screen is in a rectangular shape.

### Claim 5 (depends on 1)

The method of claim 1, wherein in the first configuration, the first screen and the second screen are in contact with each other.

### Claim 6 (depends on 1)

The method of claim 1, wherein the fluid composition further comprises non-target cells that can pass through the combined filter.

### Claim 7 (depends on 6)

The method of claim 6, wherein the fluid composition comprises human blood containing the target cells.

### Claim 8 (depends on 7)

The method of claim 7, wherein the target cells comprise at least one of white blood cells and cancer cells.

### Claim 9 (depends on 7)

The method of claim 7, wherein the pores of the first and second screens are sized and arranged to form the combined filter configured to screen at least one of white blood cells and cancer cells.

### Claim 10 (depends on 9)

The method of claim 9, wherein the pores of the first and second screens are sized and arranged to form the combined filter configured to pass red blood cells therethrough.