Compositions and methods for detecting human pegivirus 2 (HPgV-2)

A composition comprising a synthetic nucleic acid molecule,

• wherein said synthetic nucleic acid molecule comprises a nucleotide sequence at least 12 nucleotides in length that hybridizes under stringent conditions to a portion of an HPgV-2 genomic sequence selected from the group consisting of:
• the NS2 gene, the 5′ UTR, the S gene, the E1 gene, the E2 gene, the NS4a gene, the NS4b gene, and the 3′ UTR, and wherein said genomic sequence of HPgV-2 is shown in SEQ ID NO:
• 1, 75, or 299-303;
• wherein the synthetic nucleic acid molecule is linked to a fluorescent label, chemiluminescent label or enzymatic label, a heterologous nucleic acid sequence or a solid support or the synthetic nucleic acid molecule comprises at least one modified base selected from the group consisting of:
• phosphorothioate, boranophosphate, 4′-thio-ribose, locked nucleic acid, 2′-0-(2′-methoxyethyl), 2′-0-methyl, 2′-fluoro, 2′-deoxy-T-fluoro-b-D-arabinonucleic acid, phosphonoacetate, 2′-3′-seco-RNA, Morpholino nucleic acid analog, Peptide nucleic acid analog, phosphorodithioate, phosphoramidate, methylphosphonate, 4-acetylcytosine, 8-hydroxy-N6-methyladenosine, aziridinylcytosine, pseudoisocytosine, 5-(carboxyhydroxylmethyl)uracil, 5-fluorouracil, 5-bromouracil, 5-carboxymethylaminomethyl-2-thiouracil, 5-carboxymethylaminomethyluracil, dihydrouracil, inosine, N6-isopentenyladenine, 1-methyladenine, 1-methylpseudouracil, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-methyladenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxy-aminomethyl-2-thiouracil, beta-D-mannosylqueosine, 5′-methoxycarbonylmethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid, oxybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, N-uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid, pseudouracil, queosine, 2-thiocytosine, 2,6-diaminopurine, 2-aminopurine, 5-amino-allyluracil, 5-hydroxymethylcytosine, 5-iodouracil, 5-nitroindole, 5-propynylcytosine, 5-propynyluracil, hypoxanthine, N3-methyluracil, N6,N6-dimethyladenine, purine, C-5-propynyl cytosine, C-5-propynyl uracil, and difluorotouyl.