# US 10,501,770 B2 · Multiple-use renewable electrochemical sensors based on direct drawing of enzymatic inks

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- Number: US 10,501,770 B2
- Document ID: US10501770B2
- Status: Granted
- Kind: B2
- Application number: 15/548,735
- Filed: 2016-02-05
- Published: 2018-02-01
- Granted: 2019-12-10
- Earliest claimed: 2015-02-05
- Assignees: The Regents of the University of California
- Applicants: The Regents of the University of California
- Inventors: Joseph Wang; Amay Jairaj Bandodkar
- Web page: https://patentgrep.com/patent/US10501770B2

## CPC

- C12Q 1/006: {for glucose}
- A61B 5/02: Detecting, measuring or recording for evaluating the cardiovascular system, e.g. pulse, heart rate, blood pressure or blood flow
- A61B 5/14532: {for measuring glucose, e.g. by tissue impedance measurement}
- A61B 5/1486: using enzyme electrodes, e.g. with immobilised oxidase
- A61B 5/6898: {Portable consumer electronic devices, e.g. music players, telephones, tablet computers}
- C12Q 1/001: {Enzyme electrodes}
- G01N 27/3271: {Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood (amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001)}
- G01N 27/3272: {Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels (optical biosensors G01N33/52)}
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Methods, systems, and devices are disclosed for providing a portable enzymatic-ink dispensing system. The system includes an enzymatic-ink including one or more biocompatible binders, one or more biocompatible mediators, an enzyme, an enzyme stabilizer, and a conductive material. The system includes a dispenser including a chamber to hold the enzymatic-ink and an applicator to apply the enzymatic ink dispensed from the chamber onto a target substrate.

## Claims

### Claim 1

A portable apparatus comprising:
a dispenser having volume to hold and dispense one or more individually dispensable pellets made up of an enzyme compound;
a case comprising a mechanism using which the dispenser can be attached to the case; and
an electrochemical sensor attached to the case and having a reference electrode, a working electrode and a counter electrode arranged to provide a flat surface for application of the enzyme compound and a chemical for sensing and to produce an electrical signal resulting from the sensing.

### Claim 2 (depends on 1)

The portable apparatus of claim 1, wherein the case is shaped to securely hold a user device.

### Claim 3 (depends on 1)

The portable apparatus of claim 1, wherein the dispenser includes a stylus-shaped container having an elongated body with a cavity, wherein the cavity is user accessible at one end to fill the dispensable pellets.

### Claim 4 (depends on 1)

The portable apparatus of claim 1, wherein the electrochemical sensor is fabricated directly on the case.

### Claim 5 (depends on 1)

The portable apparatus of claim 1, wherein the mechanism includes an electromagnet.

### Claim 6 (depends on 1)

The portable apparatus of claim 1, further including interface electronics mounted on the case, the interface electronics having a first interface with the electrochemical sensor and a second interface with a smartphone.

### Claim 7 (depends on 6)

The portable apparatus of claim 6, wherein the second interface is a wireless interface by which the portable apparatus causes a measurement from the electrochemical sensor to be displayed in a human-readable format on a user interface of the smartphone.