# US 10,501,757 B2 · Simian adenoviruses SAdV-43, -45, -46, -47, -48, -49, and -50, and uses thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,501,757 B2 - Document ID: US10501757B2 - Status: Granted - Kind: B2 - Application number: 15/449,076 - Filed: 2017-03-03 - Published: 2018-01-18 - Granted: 2019-12-10 - Earliest claimed: 2008-10-31 - Assignees: The Trustees of the University of Pennsylvania - Applicants: The Trustees of The University of Pennsylvania - Inventors: Soumitra Roy; James M. Wilson; Luc H. Vandenberghe - Web page: https://patentgrep.com/patent/US10501757B2 ## CPC - C12N 15/86: Viral vectors - C12N 2710/10321: Viruses as such, e.g. new isolates, mutants or their genomic sequences - C12N 2710/10322: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes - C12N 2710/10341: Use of virus, viral particle or viral elements as a vector - C12N 2710/10342: virus or viral particle as vehicle, e.g. encapsulating small organic molecule - C12N 2710/10343: viral genome or elements thereof as genetic vector - C12N 2710/10345: Special targeting system for viral vectors - C12N 2710/10352: relating to complementing cells and packaging systems for producing virus or viral particles - C12N 2810/6018: Adenoviridae - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract A recombinant vector comprises simian adenovirus 43, 45, 46, 47, 48, 49 or 50 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus 43, 45, 46, 47, 48, 49 or 50 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided. ## Claims ### Claim 1 A recombinant adenovirus having an adenovirus capsid comprising a hexon protein, a penton protein, and a fiber protein, wherein said hexon protein is a hexon protein of SAdV-45 comprising the amino acids 1 to 953 of SEQ ID NO: 48; said capsid encapsidating a heterologous nucleic acid comprising a gene operably linked to expression control sequences which direct at least one of transcription, translation, and expression of the gene in a host cell. ### Claim 2 (depends on 1) The recombinant adenovirus according to claim 1, further comprising a 5′ and a 3′ adenovirus cis-element necessary for replication and encapsidation. ### Claim 3 (depends on 1) The recombinant adenovirus according to claim 1, wherein said adenovirus lacks all or a part of the E1 gene. ### Claim 4 (depends on 3) The recombinant adenovirus according to claim 3, wherein said adenovirus is replication—defective. ### Claim 5 (depends on 1) The recombinant adenovirus according to claim 1, wherein said capsid is a hybrid capsid. ### Claim 6 (depends on 5) The recombinant adenovirus according to claim 5, wherein the hybrid capsid comprises at least one capsid protein from an adenovirus selected from the group consisting of SAdV-46, SAdV-43, SAdV-47, SAdV-48, SAdV-49, and SAdV-50. ### Claim 7 (depends on 6) The recombinant adenovirus according to claim 6, wherein the penton protein is selected from: a penton protein of SAdV-46 with the amino acids 1 to 563 of SEQ ID NO: 74; a penton protein of SAdV-43 with the amino acids 1 to 651 of SEQ ID NO: 6; a penton protein of SAdV-47 with the amino acids 1 to 580 of SEQ ID NO: 104; a penton protein of SAdV-48 with the amino acids 1 to 504 of SEQ ID NO: 134; a penton protein of SAdV-49 with the amino acids 1 to 511 of SEQ ID NO: 162; and a penton protein of SAdV-50 with the amino acids 1 to 511 of SEQ ID NO: 188. ### Claim 8 (depends on 6) The recombinant adenovirus according to claim 6, wherein the fiber protein is selected from: a fiber protein of SAdV-43 with amino acids 1 to 599 of SEQ ID NO: 22; a fiber protein of SAdV-46 with amino acids 1 to 353 of SEQ ID NO: 89; a fiber protein of SAdV-47 with amino acids 1 to 322 of SEQ ID NO: 119; a fiber protein of SAdV-48 with amino acids 1 to 570 of SEQ ID NO: 147; a fiber protein of SAdV-49 with amino acids 1 to 521 of SEQ ID NO: 174; a fiber protein of SAdV-49 with amino acids 1 to 418 of SEQ ID NO: 175; a fiber protein of SAdV-50 with amino acids 1 to 521 of SEQ ID NO: 200; and a fiber protein of SAdV-50 with amino acids 1 to 418 of SEQ ID NO: 201. ### Claim 9 (depends on 1) A composition comprising the recombinant adenovirus according to claim 1 in a pharmaceutically acceptable carrier. ### Claim 10 A recombinant adenovirus having a capsid comprising a fiber protein, a penton protein, and a hexon protein, said hexon protein comprising (a) a full-length of amino acids about 51 to 953 of SEQ ID NO: 48 or (b) the full length of amino acids 1 to about 903 of SEQ ID NO: 48, said adenovirus capsid encapsidating a gene heterologous to SAdV-45 operably linked to expression control sequences which direct at least one of transcription, translation, and expression of the gene in a host cell. ### Claim 11 (depends on 10) The recombinant adenovirus according to claim 10, wherein the fiber protein is of an adenovirus selected from SAdV-46, SAdV-43, SAdV-45, SAdV-47, SAdV-48, SAdV-49, and SAdV-50. ### Claim 12 (depends on 10) The recombinant adenovirus according to claim 10, wherein the penton protein is of an adenovirus selected from SAdV-46, SAdV-43, SAdV-45, SAdV-47, SAdV-48, SAdV-49, and SAdV-50. ### Claim 13 (depends on 10) The recombinant adenovirus according to claim 10, wherein said adenovirus is a pseudotyped adenovirus comprising a 5′ and a 3′ adenovirus cis-element necessary for replication and encapsidation, said cis-elements comprising an adenovirus 5′ inverted terminal repeat and an adenovirus 3′ inverted terminal repeat. ### Claim 14 (depends on 10) The recombinant adenovirus according to claim 10, wherein the recombinant adenovirus comprises one or more adenovirus genes. ### Claim 15 (depends on 10) The recombinant adenovirus according to claim 10, wherein the recombinant adenovirus is replication-defective. ### Claim 16 (depends on 15) The recombinant adenovirus according to claim 15, wherein the recombinant adenovirus comprises a deletion in the E1 region.