# US 10,501,754 B2 · Disease resistant potato plants > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,501,754 B2 - Document ID: US10501754B2 - Status: Granted - Kind: B2 - Application number: 15/191,919 - Filed: 2016-06-24 - Published: 2016-10-13 - Granted: 2019-12-10 - Earliest claimed: 2007-02-01 - Assignees: Enza Zaden Beheer B.V. - Applicants: Enza Zaden Beheer B.V. - Inventors: Mireille Maria Augusta Van Damme; Augustinus Franciscus Johannes Maria Van Den Ackerveken; Christianus Cornelis Nicolaas Van Schie; Tieme Zeilmaker - Web page: https://patentgrep.com/patent/US10501754B2 ## CPC - C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance} - C07K 14/415: from plants - C12N 9/0071: {acting on paired donors with incorporation of molecular oxygen (1.14)} - C12N 15/8218: {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]} - C12N 15/8239: {pathogen inducible} - C12N 15/8282: {for fungal resistance} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract The present invention relates to a plant, which is resistant to a pathogen of viral, bacterial, fungal or oomycete origin, wherein the plant has a reduced level, reduced activity or complete absence of DMR6 protein as compared to a plant that is not resistant to the said pathogen, in particular organisms of the Fungi or the phylum Oomycota. The invention further relates to a method for obtaining a plant, which is resistant to a pathogen of viral, bacterial, fungal or oomycete origin, comprising reducing the endogenous level or activity of DMR6 protein in the plant. In addition, the invention relates to the use of a DMR6 promotor for providing disease resistant plants. ## Claims ### Claim 1 An isolated potato plant which is resistant to Phytophthora infestans, wherein the potato plant has reduced expression of a first DMR6 protein of SEQ ID NO: 112 as compared to a corresponding wild-type potato plant, and wherein the potato plant has a non-natural mutation introduced into a first dmr6 gene of SEQ ID NO: 113 encoding said first DMR6 protein; and wherein the potato plant has reduced expression of a second DMR6 protein of SEQ ID NO: 114 as compared to a corresponding wild-type potato plant, and wherein the potato plant has a non-natural mutation introduced into a second dmr6 gene of SEQ ID NO: 115 encoding said second DMR6 protein. ### Claim 2 (depends on 1) The potato plant as claimed in claim 1, wherein the mutation in the first dmr6 gene leads to an amino acid substitution in the first DMR6 protein and the mutation in the second dmr6 gene leads to an amino acid substitution in the second DMR6 protein. ### Claim 3 (depends on 1) The potato plant as claimed in claim 1, wherein the potato plant has a non-natural mutation introduced into the first dmr6 gene of SEQ ID NO: 113 and the potato plant has reduced expression of the first DMR6 protein of SEQ ID NO: 112. ### Claim 4 (depends on 1) A seed, tissue, or plant part of the potato plant according to claim 1, wherein the seed, tissue, or plant part comprises the mutation in the first dmr6 gene of SEQ ID NO: 113 and has reduced expression of the first DMR6 protein of SEQ ID NO: 112. ### Claim 5 (depends on 1) The potato plant as claimed in claim 1, wherein the potato plant has a non-natural mutation introduced into the second dmr6 gene of SEQ ID NO: 115 and the potato plant has reduced expression of the second DMR6 protein of SEQ ID NO: 114. ### Claim 6 (depends on 1) A seed, tissue, or plant part of the potato plant according to claim 1, wherein the seed, tissue, or plant part comprises the mutation in the second dmr6 gene of SEQ ID NO: 115 and has reduced expression of the second DMR6 protein of SEQ ID NO: 114. ### Claim 7 A method for obtaining a potato plant which is resistant to Phytophthora infestans, comprising reducing an endogenous level of a first DMR6 protein of SEQ ID NO: 112 in a potato plant by introducing a mutation into a first dmr6 gene of SEQ ID NO: 113 encoding said first DMR6 protein, and reducing an endogenous level of a second DMR6 protein of SEQ ID NO: 114 in a potato plant by introducing a mutation into a second dmr6 gene of SEQ ID NO: 115 encoding said second DMR6 protein. ### Claim 8 (depends on 7) The method according to claim 7, wherein the endogenous level of the first DMR6 protein of SEQ ID NO: 112 in the potato plant is reduced by introducing a mutation into the first dmr6 gene of SEQ ID NO: 113. ### Claim 9 (depends on 7) The method according to claim 7, wherein reducing the endogenous level of the first DMR6 protein of SEQ ID NO: 112 in the potato plant is achieved by reducing expression of the first dmr6 gene of SEQ ID NO: 113. ### Claim 10 (depends on 9) The method according to claim 9, wherein reducing expression of the first dmr6 gene of SEQ ID NO: 113 is achieved by gene silencing or RNAi. ### Claim 11 (depends on 7) The method according to claim 7, wherein the mutation results in an amino acid substitution in the first DMR6 protein of SEQ ID NO: 112. ### Claim 12 (depends on 7) The method according to claim 7, wherein the endogenous level of the second DMR6 protein of SEQ ID NO: 114 in the potato plant is reduced by introducing a mutation into the second dmr6 gene of SEQ ID NO: 115. ### Claim 13 (depends on 7) The method according to claim 7, wherein reducing the endogenous level of the second DMR6 protein of SEQ ID NO: 114 in the potato plant is achieved by reducing expression of the second dmr6 gene of SEQ ID NO: 115. ### Claim 14 (depends on 13) The method according to claim 13, wherein reducing expression of the second dmr6 gene of SEQ ID NO: 115 is achieved by gene silencing or RNAi. ### Claim 15 (depends on 7) The method according to claim 7, wherein the mutation results in an amino acid substitution in the second DMR6 protein of SEQ ID NO: 114. ### Claim 16 (depends on 7) The method according to claim 7, wherein one or more of the mutations are effected by a mutagenic treatment of the potato plant. ### Claim 17 (depends on 16) The method according to claim 16, wherein the mutagenic treatment is effected with a mutagen or with radiation. ### Claim 18 (depends on 7) An isolated potato plant produced from the method according to claim 7, wherein the potato plant comprises the mutation in the first dmr6 gene of SEQ ID NO: 113 and has reduced expression of the first DMR6 protein of SEQ ID NO: 112 as compared to a corresponding wild-type potato plant. ### Claim 19 (depends on 18) A seed, tissue, or plant part of the potato plant according to claim 18, wherein the seed, tissue, or plant part comprises the mutation in the first dmr6 gene of SEQ ID NO: 113 and has reduced expression of the first DMR6 protein of SEQ ID NO: 112. ### Claim 20 (depends on 7) An isolated potato plant produced from the method according to claim 7, wherein the potato plant comprises the mutation in the second dmr6 gene of SEQ ID NO: 115 and has reduced expression of the second DMR6 protein of SEQ ID NO: 114 as compared to a corresponding wild-type potato plant. ### Claim 21 (depends on 20) A seed, tissue, or plant part of the potato plant according to claim 20, wherein the seed, tissue, or plant part comprises the mutation in the second dmr6 gene of SEQ ID NO: 115 and has reduced expression of the second DMR6 protein of SEQ ID NO: 114. ### Claim 22 (depends on 7) An isolated potato plant produced from the method according to claim 7, wherein the potato plant comprises the mutation in the first dmr6 gene of SEQ ID NO: 113 and has reduced expression of the first DMR6 protein of SEQ ID NO: 112 as compared to a corresponding wild-type potato plant, and further comprises the mutation in the second dmr6 gene of SEQ ID NO: 115 and has reduced expression of the second DMR6 protein of SEQ ID NO: 114 as compared to a corresponding wild-type potato plant. ### Claim 23 (depends on 22) A seed, tissue, or plant part of the potato plant according to claim 22, wherein the potato plant comprises the mutation in the first dmr6 gene of SEQ ID NO: 113 and has reduced expression of the first DMR6 protein of SEQ ID NO: 112 as compared to a corresponding wild-type potato plant, and further comprises the mutation in the second dmr6 gene of SEQ ID NO: 115 and has reduced expression of the second DMR6 protein of SEQ ID NO: 114 as compared to a corresponding wild-type potato plant.