# US 10,501,752 B2 · Herbicide tolerant cotton event pDAB4468.19.10.3

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- Number: US 10,501,752 B2
- Document ID: US10501752B2
- Status: Granted
- Kind: B2
- Application number: 15/452,422
- Filed: 2017-03-07
- Published: 2017-07-06
- Granted: 2019-12-10
- Earliest claimed: 2012-01-23
- Assignees: Dow AgroSciences LLC
- Applicants: Dow AgroSciences LLC
- Inventors: Yunxing C. Cui; Raina King; Tina M. Kaiser; Andrew E. Robinson; Dayakar Pareddy; Sandra G. Toledo; Leon B. Braxton; David M. Anderson; Terry R. Wright
- Web page: https://patentgrep.com/patent/US10501752B2

## CPC

- C12N 15/8274: {for herbicide resistance}
- A01N 37/38: having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N 39/04: Aryloxy-acetic acids; Derivatives thereof
- A01N 43/40: six-membered rings
- A01N 57/20: containing acyclic or cycloaliphatic radicals
- C12N 9/0069: {acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)}
- C12N 9/0071: {acting on paired donors with incorporation of molecular oxygen (1.14)}
- C12N 15/8277: {Phosphinotricin}
- C12Q 1/6876: Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- C12Q 2600/158: Expression markers
- C12Q 2600/16: Primer sets for multiplex assays
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Cotton event pDAB4468.19.10.3 comprises genes encoding AAD-12 and PAT, affording herbicide tolerance to cotton crops containing the event, and enabling methods for crop protection.

## Claims

### Claim 1

A transgenic cotton seed comprising in its genome a pat/aad-12 transgenic event comprising a polynucleotide that is at least 99% identical to SEQ ID NO:21.

### Claim 2 (depends on 1)

The transgenic cotton seed of claim 1, wherein the polynucleotide comprises a first nucleotide sequence consisting of nucleotides 1329-1380 of SEQ ID NO:1, and a second nucleotide sequence consisting of nucleotides 143-194 of SEQ ID NO:2.

### Claim 3 (depends on 2)

The transgenic cotton seed of claim 2, wherein the polynucleotide comprises a first nucleotide sequence consisting of nucleotides 1254-1455 of SEQ ID NO:1, and a second nucleotide sequence consisting of nucleotides 118-219 of SEQ ID NO:2.

### Claim 4 (depends on 3)

The transgenic cotton seed of claim 3, wherein the polynucleotide comprises a first nucleotide sequence consisting of nucleotides 1304-1405 of SEQ ID NO:1, and a second nucleotide sequence consisting of nucleotides 68-269 of SEQ ID NO:2.

### Claim 5 (depends on 4)

The transgenic cotton seed of claim 4, wherein the polynucleotide comprises a first nucleotide sequence consisting of nucleotides 1054-1655 of SEQ ID NO:1, and a second nucleotide sequence consisting of nucleotides 1-369 of SEQ ID NO:2.

### Claim 6 (depends on 1)

The transgenic cotton seed of claim 1, wherein the polynucleotide comprises SEQ ID NO:21.

### Claim 7

A transgenic cotton plant comprising in its genome a pat/aad-12 transgenic event comprising a polynucleotide that is at least 99% identical to SEQ ID NO:21.

### Claim 8 (depends on 7)

A part of the transgenic cotton plant of claim 7, wherein the part is selected from the group consisting of pollen, ovule, flowers, bolls, shoots, roots, and leaves.

### Claim 9 (depends on 7)

A method for breeding a cotton plant, the method comprising:
crossing the transgenic cotton plant of claim 7 with a second cotton plant to produce a progeny cotton plant; and
assaying the progeny cotton plant for the presence of the pat/aad-12 transgenic event.

### Claim 10 (depends on 9)

The method according to claim 9, wherein assaying the progeny cotton plant for the presence of the pat/aad-12 transgenic event comprises amplifying genomic DNA from the progeny plant in a polymerase chain reaction or applying an herbicide to the progeny cotton plant, wherein the herbicide is a phenoxyacetic acid herbicide, a pyridoxyacetic acid herbicide, or glufosinate.

### Claim 11

A cotton meal, cotton fiber, or cotton oil from a genetically-modified plant, the meal, fiber, or oil comprising a polynucleotide that is at least 99% identical to SEQ ID NO:21.

### Claim 12 (depends on 7)

A method for controlling weeds in a cotton crop comprising the transgenic cotton plant of claim 7, the method comprising:
applying an herbicide to the cotton crop, wherein the herbicide is a phenoxyacetic acid herbicide, a pyridoxyacetic acid herbicide, or glufosinate.

### Claim 13 (depends on 12)

The method according to claim 12, wherein the herbicide is a phenoxyacetic acid herbicide.

### Claim 14 (depends on 13)

The method according to claim 13, wherein the phenoxyacetic acid herbicide is 2,4-D or MCPA.

### Claim 15 (depends on 12)

The method according to claim 12, wherein the herbicide is a pyridoxyacetic acid herbicide.

### Claim 16 (depends on 15)

The method according to claim 15, wherein the pyridoxyacetic acid herbicide is triclopyr or fluroxypyr.

### Claim 17 (depends on 12)

The method according to claim 12, wherein the herbicide is glufosinate.