# US 10,501,750 B2 · Isolated polynucleotides and polypeptides, and methods of using same for increasing plant yield and/or agricultural characteristics

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- Number: US 10,501,750 B2
- Document ID: US10501750B2
- Status: Granted
- Kind: B2
- Application number: 15/979,507
- Filed: 2018-05-15
- Published: 2018-08-30
- Granted: 2019-12-10
- Earliest claimed: 2011-08-23
- Assignees: Evogene Ltd.
- Applicants: Evogene Ltd.
- Inventors: Limor Poraty-Gavra; Eyal Emmanuel; Hagai Karchi
- Web page: https://patentgrep.com/patent/US10501750B2

## CPC

- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/158: Expression markers
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Provided are isolated polynucleotides at least 80% identical to SEQ ID NOs: 1-479, 813-5173, 8511, 8513, 8515, 8517, 8519, 8521, 8523, 9096-9141 and 9142; and isolated polypeptides at least 80% homologous to an amino acid sequence selected from the group consisting of SEQ ID NOs: 624, 480-623, 625-812, 5174-7015, 7017-7021, 7024, 7026-8510, 8512, 8514, 8516, 8518, 8520, 8522, 8524, 9143-9177, such as the polypeptides set forth in SEQ ID NO:480-812, 5174-8510, 8512, 8514, 8516, 8518, 8520, 8522, 8524, and 9143-9177, nucleic acid constructs comprising same, transgenic cells and plants expressing same and methods of using same for increasing yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, nitrogen use efficiency, and/or abiotic stress tolerance of a plant.

## Claims

### Claim 1

A method of increasing yield, biomass and/or growth rate of a transgenic plant as compared to a control plant of the same species which is grown under the same growth conditions, comprising transforming the plant with an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity and conservative amino acid substitutions with respect to SEQ ID NO: 794, thereby increasing the yield, biomass and growth rate of the plant.

### Claim 2 (depends on 1)

The method of claim 1, wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs: 794, 649 and 7523-7527.

### Claim 3 (depends on 1)

The method of claim 1, wherein said amino acid sequence is expressed from a polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 436, 170 and 3976-3981.

### Claim 4

A method of selecting a transformed plant, comprising:
(a) providing transgenic plants transformed with an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide having at least 95% sequence identity and conservative amino acid substitutions with respect to the amino acid sequence of SEQ ID NO: 794 and
(b) selecting said plants for an increased yield, biomass and/or growth rate as compared to a control plant of the same species which is grown under the same growth conditions, and/or for a reduced time to flowering as compared to a control plant of the same species which is grown under the same growth conditions,
thereby selecting the plant having increased yield, biomass and growth rate as compared to the control plant of the same species which is grown under the same growth conditions and/or a reduced time to flowering as compared to a control plant of the same species which is grown under the same growth conditions.

### Claim 5 (depends on 4)

The method of claim 4, wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs: 794, 649 and 7523-7527.

### Claim 6 (depends on 4)

The method of claim 4, wherein said amino acid sequence is expressed from a polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 436, 170 and 3976-3981.

### Claim 7 (depends on 1)

The method of claim 1, wherein the yield comprises seed yield.

### Claim 8 (depends on 1)

The method of claim 1, wherein said amino acid sequence is SEQ ID NO: 794.