# US 10,501,745 B2 · Promoter and use thereof

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- Number: US 10,501,745 B2
- Document ID: US10501745B2
- Status: Granted
- Kind: B2
- Application number: 15/563,750
- Filed: 2016-03-11
- Published: 2018-04-05
- Granted: 2019-12-10
- Earliest claimed: 2015-04-02
- Assignees: Cj Cheiljedang Corporation
- Applicants: Cj Cheiljedang Corporation
- Inventors: Young Mi Lee; Seung Bin Lee; Seong Bo Kim; Ji Hyun Lee; Jin Sook Chang; Seung Hyun Cho; Seung Won Park
- Web page: https://patentgrep.com/patent/US10501745B2

## CPC

- C12N 15/70: Vectors or expression systems specially adapted for E. coli
- C12N 1/205: Bacterial isolates
- C12N 15/63: Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N 15/77: for Corynebacterium; for Brevibacterium
- C12P 19/02: Monosaccharides (2-ketogulonic acid C12P7/60)
- C12P 21/00: Preparation of peptides or proteins (single cell protein C12N1/00)
- C12P 21/02: having a known sequence of two or more amino acids, e.g. glutathione
- C07H 21/04: with deoxyribosyl as saccharide radical
- C12N 15/74: Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12R 2001/15: Corynebacterium
- C12R 2001/19: Escherichia coli
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present disclosure relates to a novel promoter, a vector comprising the promoter, a microorganism comprising the vector, and a method for producing a target protein using the promoter.

## Claims

### Claim 1

An isolated nucleic acid molecule having a promoter activity, wherein the isolated nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 1.

### Claim 2 (depends on 1)

A target protein expression cassette comprising the isolated nucleic acid molecule having a promoter activity of claim 1 and a gene encoding a target protein.

### Claim 3 (depends on 1, 2)

A recombinant vector comprising the isolated nucleic acid molecule of claim 1 or the target protein expression cassette of claim 2.

### Claim 4 (depends on 3)

The recombinant vector of claim 3, wherein the target protein is a psicose-3-epimeras.

### Claim 5 (depends on 4)

A method for producing psicose, comprising:
(a) culturing the microorganism introduced with the vector of claim 4; and
(b) reacting the cultured microorganism with fructose to produce psicose.

### Claim 6 (depends on 4)

A recombinant microorganism introduced with the vector of claim 4.

### Claim 7 (depends on 6)

The recombinant microorganism of claim 6, wherein the microorganism is a microorganism of the genus Corynebacterium.

### Claim 8 (depends on 7)

The recombinant microorganism of claim 7, wherein the microorganism of the genus Corynebacterium is Corynebacterium glutamicum or Corynebacterium ammoniagenes.

### Claim 9 (depends on 6)

The recombinant microorganism of claim 6, wherein the microorganism is a microorganism of the genus Escherichia.

### Claim 10 (depends on 9)

The recombinant microorganism of claim 9, wherein the microorganism of the genus Escherichia is Escherichia coli.

### Claim 11 (depends on 6)

A method for producing a target protein, comprising:
(a) culturing the recombinant microorganism of claim 6 in a medium to produce a target protein; and
(b) recovering the produced target protein from the cultured microorganism or the medium.

### Claim 12 (depends on 3)

A recombinant microorganism introduced with the vector of claim 3.

### Claim 13 (depends on 12)

The recombinant microorganism of claim 12, wherein the microorganism is a microorganism of the genus Corynebacterium.

### Claim 14 (depends on 13)

The recombinant microorganism of claim 13, wherein the microorganism of the genus Corynebacterium is Corynebacterium glutamicum or Corynebacterium ammoniagenes.

### Claim 15 (depends on 12)

The recombinant microorganism of claim 12, wherein the microorganism is a microorganism of the genus Escherichia.

### Claim 16 (depends on 15)

The recombinant microorganism of claim 15, wherein the microorganism of the genus Escherichia is Escherichia coli.

### Claim 17 (depends on 12)

A method for producing a target protein, comprising:
(a) culturing the recombinant microorganism of claim 12 in a medium to produce the target protein; and
(b) recovering the produced target protein from the cultured microorganism or the medium.