# US 10,501,736 B2 · Solid matrix for the storage of biological samples

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,501,736 B2
- Document ID: US10501736B2
- Status: Granted
- Kind: B2
- Application number: 14/843,262
- Filed: 2015-09-02
- Published: 2016-03-03
- Granted: 2019-12-10
- Earliest claimed: 2013-03-15
- Assignees: Ge Healthcare UK Limited
- Applicants: Ge Healthcare UK Limited
- Inventors: Hugh O'Malley; Barry Johnson
- Web page: https://patentgrep.com/patent/US10501736B2

## CPC

- C12N 15/1006: {by means of a solid support carrier, e.g. particles, polymers}
- C07H 21/00: Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C12N 15/1017: {by filtration, e.g. using filters, frits, membranes}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13)

## Abstract

The present invention relates to a method for storage and subsequent lysis of a sample in which the sample is immobilized on a solid support. The solid matrix is embedded with a low concentration of both a chaotropic salt and a surfactant which act synergistically to efficiently store and lyse a biological sample.

## Claims

### Claim 1

A solid matrix for storing and/or lysing a biological sample comprising a surfactant and a chaotropic salt, wherein the concentration of the chaotropic salt is greater than 0.05M and less than 0.5M.

### Claim 2 (depends on 1)

The solid matrix of claim 1, wherein the surfactant concentration is greater than 0.05M and less than 0.5M.

### Claim 3 (depends on 1)

The solid matrix of claim 1, wherein the chaotropic salt and surfactant is present in a ratio of 1:1.

### Claim 4 (depends on 1)

The solid matrix of claim 1, wherein the chaotropic salt and surfactant is present in a ratio of 1:10.

### Claim 5 (depends on 1)

The solid matrix of claim 1, wherein the concentration of chaotropic salt to surfactant is selected from the group consisting of 0.01M:01M, 0.1M:0.01M, 0.05M:0.05M, 0.1M:0.1M, 0.2M:0.2M, 0.3M:0.3M and 0.4M:0.4M.

### Claim 6 (depends on 1)

The solid matrix of claim 1, wherein said surfactant is Triton X100, and the chaotropic salt is a guanidinium salt.

### Claim 7 (depends on 1)

The solid matrix of claim 1, wherein the chaotropic salt is selected from the group consisting of guanidine hydrochloride, guanidine isothiocyanate and guanidine thiocyanate.

### Claim 8 (depends on 1)

The solid matrix of claim 1, wherein the chaotropic salt is guanidine thiocyanate.

### Claim 9 (depends on 1)

The solid matrix of claim 1, wherein the biological sample is selected from the group consisting of a cellular sample, blood, serum, semen, cerebral spinal fluid, synovial fluid, lymphatic fluid, saliva, buccal, cervical cell, vaginal cell, urine, faeces, hair, skin and muscle, and
wherein the solid matrix is selected from the group consisting of alginate, glass, glass fiber, glass microfiber, silica, silica gel, silica oxide, cellulose, nitrocellulose, carboxymethylcellulose, polyester, polyamide, carbohydrate polymers, polypropylene, polytetraflurorethylene, polyvinylidinefluoride, wool and porous ceramics.

### Claim 10 (depends on 1)

The solid matrix of claim 1, wherein the solid matrix is a cellulose based matrix, and said cellulose based matrix is in the form of a pre punched disc.

### Claim 11 (depends on 1)

A method of storing and/or lysing a biological sample comprising the steps:
i) contacting the solid matrix of claim 1 with a biological sample; and
ii) storing said solid matrix with said biological sample, wherein the chaotropic salt and surfactant have a synergistic effect in lysing the cell.

### Claim 12 (depends on 11)

The method of claim 11, further comprising a step iii) of recovering the cell lysate from the solid matrix.

### Claim 13 (depends on 11)

The method of claim 11, wherein the biological sample is selected from a group consisting of a cellular sample, blood, serum, semen, cerebral spinal fluid, synovial fluid, lymphatic fluid, saliva, buccal, cervical cell, vaginal cell, urine, faeces, hair, skin and muscle.

### Claim 14 (depends on 1)

A kit for storing and amplifying nucleic acid comprising the solid matrix of claim 1 and instructions for use thereof.

### Claim 15 (depends on 1)

The solid matrix of claim 1, wherein the chaotropic salt and surfactant are present in a molar ratio in a range from 1:10 to 10:1, inclusive.