# US 10,501,735 B2 · Device with controlled fluid dynamics, for isolation of an analyte from a sample

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- Number: US 10,501,735 B2
- Document ID: US10501735B2
- Status: Granted
- Kind: B2
- Application number: 13/972,788
- Filed: 2013-08-21
- Published: 2014-02-27
- Granted: 2019-12-10
- Earliest claimed: 2012-08-23
- Assignees: Quidel Corporation; Northwestern University
- Applicants: Northwestern University; Quidel Corporation
- Inventors: David M. Kelso; Kunal Sur; Tom Westberg; Zaheer Parpia; Mark J. Fisher
- Web page: https://patentgrep.com/patent/US10501735B2

## CPC

- C12N 15/1006: {by means of a solid support carrier, e.g. particles, polymers}
- B01L 3/502753: {characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation}
- B01L 3/502761: {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules}
- B01L 2300/0851: Bottom walls
- B01L 2300/087: Multiple sequential chambers
- Y10T 436/25375: Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

Devices for use in extracting an analyte of interest from a sample are described. In one embodiment, a device is comprised of a first plurality of chambers, where one or more chambers in the plurality of chambers has a deep end and a shallow end with a depth d1. A channel disposed between at least two adjacent chambers in the plurality of chambers has a depth greater than d1. The dimensions of the chamber and channel provide control of fluid movement in the device, particularly when introducing fluid into the device for its use and during use of the device.

## Claims

### Claim 1

A device, comprising
a first plurality of chambers disposed along a longitudinal axis of the device, one or more chambers in the first plurality of chambers having both a deep end and a shallow end arranged orthogonally to the longitudinal axis, the shallow end with a depth d1, wherein the deep end is adjacent to a side of the chambers; and
a channel disposed between and in fluid communication with at least two adjacent chambers in the first plurality of chambers, the channel having a depth greater than d1.

### Claim 2 (depends on 1)

The device of claim 1, wherein the chambers in the first plurality of chambers are serially positioned and separated by a channel.

### Claim 3 (depends on 2)

The device of claim 2, wherein the first plurality of chambers is comprised of at least three chambers, wherein two chambers in the first plurality have a deep end and a shallow end arranged orthogonally to the longitudinal axis, the shallow end with a depth d1, and wherein the channel is disposed between the two chambers having the shallow end with a depth d1.

### Claim 4 (depends on 2)

The device of claim 2, wherein the first plurality of chambers is comprised of at least five chambers, wherein three chambers in the first plurality have a deep end and a shallow end arranged orthogonally to the longitudinal axis, the shallow end with a depth d1, and wherein the channel is disposed between two chambers having the shallow end with a depth d1.

### Claim 5 (depends on 1)

The device of claim 1, wherein each chamber in the first plurality of chambers has a volume, and wherein the volume of one chamber in the plurality of chambers differs from the volume of another chamber in the first plurality.

### Claim 6 (depends on 1)

The device of claim 1, wherein the one or more chambers in the first plurality of chambers has a floor and a side wall that meet to define four corners, wherein at least the corners in the deep end of the chamber are rounded.

### Claim 7 (depends on 1)

The device of claim 1, wherein the one or more chambers in the first plurality of chambers has a floor and a side wall that meet to define four corners, wherein at least the corners in the shallow end of the chamber are angled.

### Claim 8 (depends on 1)

The device of claim 1, further comprising a cover, the cover having an access port in fluid communication with a chamber in the first plurality of chambers.

### Claim 9 (depends on 8)

The device of claim 8, wherein the access port is in fluid communication with the one or more chambers in the first plurality of chambers and wherein the access port is positioned for introduction of fluid directly into the deep end of the chamber.

### Claim 10 (depends on 1)

The device of claim 1, further comprising:
a second plurality of chambers, one or more chambers in the second plurality of chambers having a deep end and a shallow end arranged orthogonally to the longitudinal axis, the shallow end with a depth d1; and
a channel disposed between at least two adjacent chambers in the second plurality of chambers, the channel having a depth greater than d1.

### Claim 11 (depends on 10)

The device of claim 10, wherein the first and second plurality of chambers are linearly parallel.