# US 10,501,721 B2 · Biological and algae harvesting and cultivation systems and methods

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- Number: US 10,501,721 B2
- Document ID: US10501721B2
- Status: Granted
- Kind: B2
- Application number: 15/273,575
- Filed: 2016-09-22
- Published: 2017-11-09
- Granted: 2019-12-10
- Earliest claimed: 2016-05-09
- Assignees: Global Algae Technologies, LLC
- Applicants: Global Algae Innovations, Inc.
- Inventors: David A. Hazlebeck; William Rickman; Rodney Corpuz
- Web page: https://patentgrep.com/patent/US10501721B2

## CPC

- C12N 1/12: Unicellular algae; Culture media therefor (as new plants A01H13/00)
- B01D 61/22: Controlling or regulating
- B01D 63/02: Hollow fibre modules
- B01D 63/04: comprising multiple hollow fibre assemblies
- B01D 63/046: {in separate housings}
- B01D 65/02: Membrane cleaning or sterilisation {; Membrane regeneration}
- C12M 21/02: {Photobioreactors (culturing algae A01G33/00, A01H4/001, C12N1/12)}
- C12M 29/04: {Filters; Permeable or porous membranes or plates, e.g. dialysis}
- C12M 29/16: {Hollow fibers (hollow fiber modules in general B01D63/02)}
- C12M 29/18: {External loop; Means for reintroduction of fermented biomass or liquid percolate (loop type reactors for chemical or physical processes B01J19/2435)}
- C12M 29/20: {Degassing; Venting; Bubble traps (means for collection or storage of gas C12M23/36; gas collection apparatus for laboratory use B01L5/02)}
- C12M 33/14: {with filters, sieves or membranes}
- C12M 41/32: {of substances in solution}
- C12M 41/44: {of volume or liquid level}
- C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)}
- C12M 45/00: {Means for pre-treatment of biological substances}
- C12M 47/02: {Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02)}
- C12N 1/02: Separating microorganisms from their culture media
- B01D 2311/04: Specific process operations in the feed stream; Feed pretreatment
- B01D 2311/06: Specific process operations in the permeate stream
- B01D 2311/2626: Absorption or adsorption
- B01D 2311/2688: Biological processes
- B01D 2313/18: Specific valves
- B01D 2313/26: Specific gas distributors or gas intakes
- B01D 2313/501: Permeate storage tanks
- B01D 2315/06: Submerged-type; Immersion type
- B01D 2315/08: Fully permeating type; Dead-end filtration
- B01D 2317/02: Elements in series
- B01D 2317/022: Reject series
- B01D 2321/04: Backflushing
- B01D 2321/18: Use of gases
- B01D 2321/185: Aeration
- B01D 2321/40: Automatic control of cleaning processes
- Y02A 40/80: in fisheries management
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Algae harvesting and cultivating systems and methods for producing high concentrations of algae product with minimal energy. In an embodiment, a dead-end filtration system and method includes at least one tank and a plurality hollow fiber membranes positioned in the at least one tank. An algae medium is pulled through the hollow fiber membranes such that a retentate and a permeate are produced.

## Claims

### Claim 1

An algae harvesting and cultivation system comprising:
a first algae cultivator defining a first cultivation stage, the first cultivation stage including a first at least one cultivation device having a first algae cultivation media for cultivating algae;
a first dead-end filtration system in fluid communication with the first cultivation stage such that the first dead-end filtration system receives at least a portion of the algae cultivated from the first cultivation stage, the first dead-end filtration system having a first plurality of hollow fiber membranes and configured to dead-end filter the algae received from the first cultivation stage through the first plurality of hollow fiber membranes so as to produce a first retentate and a first permeate;
wherein the system is constructed and arranged such that the first permeate flows back to the first cultivation stage;
a first holding reservoir constructed and arranged to receive the first permeate from the first dead-end filtration system prior to the first permeate flowing back to the first cultivation stage;
a first carbon dioxide adder configured to add carbon dioxide to the first permeate prior to the first permeate flowing to the first cultivation stage;
a second algae cultivator defining a second cultivation stage, the second cultivation stage in fluid communication with the first dead-end filtration system such that the second cultivation stage receives the first retentate, the second cultivation stage including a second at least one cultivation device having a second media for cultivating additional algae using the first retentate;
a second dead-end filtration system in fluid communication with the second cultivation stage such that the second dead-end filtration system receives at least a portion of said additional algae cultivated in the second cultivation stage, the second dead-end filtration system having a second plurality of hollow fiber membranes and configured to dead-end filter the additional algae received from the second cultivation stage so as to produce a second permeate and a second retentate;
wherein the system is further constructed and arranged such that the second permeate flows back to the second cultivation stage;
a second holding reservoir constructed and arranged to receive the second permeate from the second dead-end filtration system prior to the second permeate flowing back to the second cultivation stage; and
a second carbon dioxide adder configured to add carbon dioxide to the second permeate prior to the second permeate flowing to the second cultivation stage.

### Claim 2 (depends on 1)

The algae harvesting and cultivation system of claim 1, which includes at least one of (i) a pump configured to pump said at least a portion of the algae from the first cultivation stage to the dead-end filtration system, (ii) a pump configured to pump the first permeate back to the first cultivation stage, (iii) a pump configured to pump said at least a portion of the additional algae from the second cultivation stage to the second dead-end filtration system, or (iv) a pump configured to pump the second permeate back to the second filtration stage.

### Claim 3 (depends on 1)

The algae harvesting and cultivation system of claim 1, which includes the system is constructed and arranged such that at least one of (i) gravity causes said at least a portion of the algae from the first cultivation stage to the dead-end filtration system, (ii) gravity causes the first permeate to flow back to the first cultivation stage, (iii) gravity causes said at least a portion of the additional algae from the second cultivation stage to flow to the second dead-end filtration system, or (iv) gravity causes the second permeate to flow back to the second filtration stage.

### Claim 4 (depends on 1)

The algae harvesting and cultivation system of claim 1, wherein (a) the first at least one cultivation device includes at least one of (i) an open raceway, (ii) a closed photobioreactor, (iii) an algal biofilm reactor, or (iv) a heterotrophic reactor, and (b) the second at least one cultivation device includes at least one of (i) an open raceway, (ii) a closed photobioreactor, (iii) an algal biofilm reactor, or (iv) a heterotrophic reactor.

### Claim 5 (depends on 1)

The algae harvesting and cultivation system of claim 1, wherein the system is configured such that the algae concentration of the first retentate is at least twenty times greater than the concentration of the algae cultivated in the first cultivation stage.

### Claim 6 (depends on 1)

The algae harvesting an cultivation system of claim 1, wherein the first dead-end filtration system is constructed and arranged to receive a rinse fluid, and the system is further configured to (i) stop the flow of the algae from the first cultivation stage to the first-dead end filtration system, (ii) allow the rinse fluid to flow to the first dead-end filtration system, (iii) stop the rinse fluid from flowing to the first-dead end filtration system, and (iv) allow the rinsed retentate in the first dead-end filtration system to flow to the second cultivation stage.

### Claim 7 (depends on 1)

The algae harvesting and cultivating system of claim 1, wherein the first dead-end filtration stage includes a plurality of filtration stages, and the second dead-end filtration stage includes a plurality of filtration stages.

### Claim 8 (depends on 1)

The algae harvesting and cultivating system of claim 1, which includes a pretreatment system constructed and arranged to at least one of physically or chemically change the first retentate prior to the first retentate flowing to the second cultivation stage.

### Claim 9 (depends on 8)

The algae harvesting and cultivation system of claim 8, wherein the chemical change includes at least one of: (i) a change in pH, (ii) a change in bicarbonate concentration, (iii) a change in carbonate concentration, (iv) an addition of ozone, (v) an addition of bleach, (vi) an addition of a peroxide, (vii) an addition of a chemical trigger that causes a change in cell metabolism, (viii) an addition of a herbicide, (ix) an addition of a pesticide, (x) an addition of a fungicide, (xi) an addition of a heavy metal ion, or (xii) an exposure to fluid that was used to cultivate algae predators.

### Claim 10 (depends on 8)

The algae harvesting and cultivation system of claim 8, wherein the physical change includes at least one of: (i) a change in salinity (ii) a change in osmotic strength, (iii) an exposure to UV light, (iv) an exposure to sonication, (v) an exposure to ultrasound, (vi) an exposure to cavitation, (vii) an exposure to shear forces, or (viii) a change in temperature.

### Claim 11

An algae harvesting and cultivation system comprising:
an algae cultivator including at least one cultivation device having a cultivation media for growing algae to produce an algae slurry;
at least one treatment tank defining a plurality of filtration stages including at least a first filtration stage and a second filtration stage, the first filtration stage in fluid communication with the algae cultivator such that the first filtration stage receives the algae slurry;
a first holding reservoir constructed and arranged to receive the permeate from the first filtration stage and the second filtration stage prior to the permeate flowing to the algae cultivator;
a second holding reservoir constructed and arranged to receive the permeate from the packed bed absorption column, the second holding reservoir including a carbon dioxide containing cover;
wherein the system is constructed and arranged such that the permeate can flow from the second holding reservoirs back to the algae cultivator;
wherein the first filtration stage includes:
a first at least one membrane filtration module positioned inside the at least one first treatment tank, said first at least one membrane filtration module including a first plurality of hollow fiber membranes having a first total outside filtration area and defining lumens,
a retentate outlet, and
a permeate outlet;
wherein the second filtration stage includes:
a second at least one membrane filtration module positioned inside the at least one treatment tank, said second at least one membrane filtration module including a second plurality of hollow fiber membranes having a second total outside filtration area and defining lumens,
an inlet coupled fluidly to the retentate outlet of the first filtration stage,
a retentate outlet, and
a permeate outlet; and
a nutrient adder structured and arranged to allow nutrients to be added to the permeate;
wherein the nutrient adder includes:
a packed bed absorption column in fluid communication with the holding reservoir so as to receive the permeate from the holding reservoir prior to the permeate flowing back to the algae cultivator, the packed bed absorption column including a gas stream inlet for receiving a carbon dioxide containing gas, wherein the nutrients include carbon dioxide, and wherein the carbon dioxide is added to the permeate via absorption of carbon dioxide into the permeate, the absorbed carbon dioxide converting carbonate ions in the permeate to bicarbonate ions; and
wherein the system is configured to cultivate algae and perform dead-end filtration of the algae slurry by:
(i) allowing the algae slurry to flow from the algae cultivator to the first filtration stage,
(ii) pulling permeate through pores of the first plurality of hollow fiber membranes at a first flux so that a first retentate is produced outside the lumens of the first plurality of hollow fiber membranes,
(iii) allowing at least a portion of the first retentate to flow from the first filtration stage retentate outlet to the second filtration stage retentate inlet,
(iv) pulling permeate through pores of the second plurality of hollow fiber membranes at a second flux so that a second retentate is produced outside the lumens of the second plurality of hollow fiber membranes, and
(v) allowing the permeate from the first and second filtration stages to flow back to the algae cultivator for use in the cultivation media for growing algae.

### Claim 12 (depends on 11)

The algae harvesting and cultivation system of claim 11, which includes at least one of (i) a pump configured to pump the algae slurry from the algae cultivator to the at least one treatment tank, or (ii) a pump configured to pump the permeate from the first filtration stage and the second filtration stage to the algae cultivator.

### Claim 13 (depends on 11)

The algae harvesting and cultivating system of claim 11, which includes at least one of (i) the system is constructed and arranged such that gravity causes the algae slurry from the algae cultivator to flow to the at least one treatment tank, or (ii) the system is constructed and arranged such that gravity cause the permeate from first and second filtration stages to flow to the algae cultivator.

### Claim 14 (depends on 11)

The algae harvesting and cultivation system of claim 11, wherein the at least one cultivation device includes at least one of (i) an open raceway, (ii) a closed photobioreactor, (iii) an algal biofilm reactor, or (iv) a heterotrophic reactor.

### Claim 15 (depends on 11)

The algae harvesting and cultivation system of claim 11, which includes a nutrient adder that allows carbon dioxide to be added to the permeate prior to the permeate flowing back to the algae cultivator.

### Claim 16 (depends on 11)

The algae harvesting and cultivation system of claim 11, which includes (a) a rinse fluid inlet coupled fluidly with the second filtration stage and (b) a media sensor configured to sense a concentration of media in the second filtration stage; and
wherein the system is further configured to perform a rinse sequence in which:
(i) the first retentate stops flowing from the second filtration stage, and
(ii) the rinse fluid flows to the second filtration stage until the at least one media sensor senses that at least ninety percent of the media has been replaced with the rinse fluid.

### Claim 17 (depends on 16)

The algae harvesting and cultivation system of claim 16, wherein the permeate produced during the rinse sequence does not flow back to the algae cultivator.

### Claim 18 (depends on 16)

The algae harvesting and cultivation system of claim 16, wherein the permeate produced during the rinse sequence flows back to the algae cultivator.

### Claim 19 (depends on 16)

The algae harvesting and cultivating system of claim 16, wherein the media sensor includes at least one of (i) a conductivity meter, (ii) a pH meter, or (iii) a flow meter.

### Claim 20

An algae harvesting and cultivation system comprising:
a first algae cultivator defining a first cultivation stage, the first cultivation stage including a first at least one cultivation device having a first algae cultivation media for cultivating algae;
a first dead-end filtration system in fluid communication with the first cultivation stage such that the first dead-end filtration system receives at least a portion of the algae cultivated from the first cultivation stage, the first dead-end filtration system having a first plurality of hollow fiber membranes and configured to dead-end filter the algae received from the first cultivation stage through the first plurality of hollow fiber membranes so as to produce a first retentate and a first permeate;
wherein the system is constructed and arranged such that the first permeate flows back to the first cultivation stage;
a first holding reservoir constructed and arranged to receive the first permeate from the first dead-end filtration system prior to the first permeate flowing back to the first cultivation stage;
a first carbon dioxide adder configured to add carbon dioxide to the first permeate prior to the first permeate flowing to the first cultivation stage, the first carbon dioxide adder including a first carbon dioxide absorber that enables the first permeate to absorb carbon dioxide and convert carbonate ions in the first permeate to bicarbonate ions;
a second algae cultivator defining a second cultivation stage, the second cultivation stage in fluid communication with the first dead-end filtration system such that the second cultivation stage receives the first retentate, the second cultivation stage including a second at least one cultivation device having a second media for cultivating additional algae using the first retentate;
a second dead-end filtration system in fluid communication with the second cultivation stage such that the second dead-end filtration system receives at least a portion of said additional algae cultivated in the second cultivation stage, the second dead-end filtration system having a second plurality of hollow fiber membranes and configured to dead-end filter the additional algae received from the second cultivation stage so as to produce a second permeate and a second retentate;
wherein the system is further constructed and arranged such that the second permeate flows back to the second cultivation stage;
a second holding reservoir constructed and arranged to receive the second permeate from the second dead-end filtration system prior to the second permeate flowing back to the second cultivation stage; and
a second carbon dioxide adder configured to add carbon dioxide to the second permeate prior to the second permeate flowing to the second cultivation stage, the second carbon dioxide adder including a second carbon dioxide absorber that enables the second permeate to absorb carbon dioxide and convert carbonate ions in the second permeate to bicarbonate ions.

### Claim 21

An algae harvesting and cultivation system comprising:
a first algae cultivator defining a first cultivation stage, the first cultivation stage including a first at least one cultivation device having a first algae cultivation media for cultivating algae;
a first dead-end filtration system in fluid communication with the first cultivation stage such that the first dead-end filtration system receives at least a portion of the algae cultivated from the first cultivation stage, the first dead-end filtration system having a first plurality of hollow fiber membranes and configured to dead-end filter the algae received from the first cultivation stage through the first plurality of hollow fiber membranes so as to produce a first retentate and a first permeate;
wherein the system is constructed and arranged such that the first permeate flows back to the first cultivation stage;
a first holding reservoir constructed and arranged to receive the first permeate from the first dead-end filtration system prior to the first permeate flowing back to the first cultivation stage;
a first carbon dioxide adder configured to add carbon dioxide to the first permeate prior to the first permeate flowing to the first cultivation stage, the first carbon dioxide adder including a first carbon dioxide absorber that enables the first permeate to absorb carbon dioxide and convert carbonate ions in the first permeate to bicarbonate ions, the first carbon dioxide absorber including a packed bed absorption column in fluid communication with the first holding reservoir so as to receive the first permeate from the first holding reservoir prior to the first permeate flowing back to the first cultivation stage, the first packed bed absorption column including a gas stream inlet for receiving a carbon dioxide containing gas;
a second algae cultivator defining a second cultivation stage, the second cultivation stage in fluid communication with the first dead-end filtration system such that the second cultivation stage receives the first retentate, the second cultivation stage including a second at least one cultivation device having a second media for cultivating additional algae using the first retentate;
a second dead-end filtration system in fluid communication with the second cultivation stage such that the second dead-end filtration system receives at least a portion of said additional algae cultivated in the second cultivation stage, the second dead-end filtration system having a second plurality of hollow fiber membranes and configured to dead-end filter the additional algae received from the second cultivation stage so as to produce a second permeate and a second retentate;
wherein the system is further constructed and arranged such that the second permeate flows back to the second cultivation stage;
a second holding reservoir constructed and arranged to receive the second permeate from the second dead-end filtration system prior to the second permeate flowing back to the second cultivation stage;
a second carbon dioxide adder configured to add carbon dioxide to the second permeate prior to the second permeate flowing to the second cultivation stage, the second carbon dioxide adder including a second carbon dioxide absorber that enables the second permeate to absorb carbon dioxide and convert carbonate ions in the second permeate to bicarbonate ions, the second carbon dioxide adder including a packed bed absorption column in fluid communication with the second holding reservoir so as to receive the second permeate from the second holding reservoir prior to the second permeate flowing back to the second cultivation stage, the second packed bed absorption column including a gas stream inlet for receiving a carbon dioxide containing gas;
a third holding reservoir constructed and arranged to receive the first permeate from the first packed bed absorption column, the third holding reservoir including a carbon dioxide retaining cover, wherein the system is constructed and arranged such that the first permeate flows from the third holding reservoir back to the first cultivation stage; and
a fourth holding reservoir constructed and arranged to receive the second permeate from the second packed bed absorption column, the fourth holding reservoir including a carbon dioxide retaining cover, wherein the system is constructed and arranged such that the second permeate can flow from the fourth holding reservoir back to the second cultivation stage.

### Claim 22

An algae harvesting and cultivation system comprising:
a first algae cultivator defining a first cultivation stage, the first cultivation stage including a first at least one cultivation device having a first algae cultivation media for cultivating algae;
a first dead-end filtration system in fluid communication with the first cultivation stage such that the first dead-end filtration system receives at least a portion of the algae cultivated from the first cultivation stage, the first dead-end filtration system having a first plurality of hollow fiber membranes and configured to dead-end filter the algae received from the first cultivation stage through the first plurality of hollow fiber membranes so as to produce a first retentate and a first permeate;
wherein the system is constructed and arranged such that the first permeate flows back to the first cultivation stage;
a first holding reservoir constructed and arranged to receive the first permeate from the first dead-end filtration system prior to the first permeate flowing back to the first cultivation stage;
a first carbon dioxide adder configured to add carbon dioxide to the first permeate prior to the first permeate flowing to the first cultivation stage, the first carbon dioxide adder including a first carbon dioxide absorber that enables the first permeate to absorb carbon dioxide and convert carbonate ions in the first permeate to bicarbonate ions, the first carbon dioxide absorber including a packed bed absorption column in fluid communication with the first holding reservoir so as to receive the first permeate from the first holding reservoir prior to the first permeate flowing back to the first cultivation stage, the first packed bed absorption column including a gas stream inlet for receiving a carbon dioxide containing gas;
a second algae cultivator defining a second cultivation stage, the second cultivation stage in fluid communication with the first dead-end filtration system such that the second cultivation stage receives the first retentate, the second cultivation stage including a second at least one cultivation device having a second media for cultivating additional algae using the first retentate;
a second dead-end filtration system in fluid communication with the second cultivation stage such that the second dead-end filtration system receives at least a portion of said additional algae cultivated in the second cultivation stage, the second dead-end filtration system having a second plurality of hollow fiber membranes and configured to dead-end filter the additional algae received from the second cultivation stage so as to produce a second permeate and a second retentate;
wherein the system is further constructed and arranged such that the second permeate flows back to the second cultivation stage;
a second holding reservoir constructed and arranged to receive the second permeate from the second dead-end filtration system prior to the second permeate flowing back to the second cultivation stage;
a second carbon dioxide adder configured to add carbon dioxide to the second permeate prior to the second permeate flowing to the second cultivation stage, the second carbon dioxide adder including a second carbon dioxide absorber that enables the second permeate to absorb carbon dioxide and convert carbonate ions in the second permeate to bicarbonate ions, the second carbon dioxide adder including a packed bed absorption column in fluid communication with the second holding reservoir so as to receive the second permeate from the second holding reservoir prior to the second permeate flowing back to the second cultivation stage, the second packed bed absorption column including a gas stream inlet for receiving a carbon dioxide containing gas;
a third holding reservoir constructed and arranged to receive the first permeate from the first packed bed absorption column, the third holding reservoir including a carbon dioxide retaining cover, wherein the system is constructed and arranged such that the first permeate flows from the third holding reservoir back to the first cultivation stage;
a pump configured to pump the first permeate from the third holding reservoir back to the first cultivation stage;
a fourth holding reservoir constructed and arranged to receive the second permeate from the second packed bed absorption column, the fourth holding reservoir including a carbon dioxide retaining cover, wherein the system is constructed and arranged such that the second permeate can flow from the fourth holding reservoir back to the second cultivation stage; and
a pump configured to pump the second permeate from the fourth holding reservoir back to the second cultivation stage.